Genotype influences in vivo dopamine transporter availability in human striatum

Andreas Heinz; David Goldman; Douglas W. Jones; Roberta Palmour; Dan Hommer; Julia G. Gorey; Kan S. Lee; Markku Linnoila; Daniel R. Weinberger

doi:10.1016/S0893-133X(99)00099-8

Genotype influences in vivo dopamine transporter availability in human striatum

Andreas Heinz, David Goldman, Douglas W. Jones, Roberta Palmour, Dan Hommer, Julia G. Gorey, Kan S. Lee, Markku Linnoila, Daniel R. Weinberger

Research output: Contribution to journal › Article › peer-review

476 Scopus citations

Abstract

In vivo availability of striatal dopamine transporter (DAT) protein has been reported to be reduced among alcoholics, and allelic variation of the DAT gene (SLC6A3) has been associated with severity of alcohol withdrawal. We examined the VNTR polymorphism of the 3' untranslated region of SLC6A3 and DAT protein availability in 14 abstinent alcoholics and 11 control subjects. Single photon emission computed tomography (SPECT) and plasma levels of the radioligand [I-123]β-CIT were used to quantify DAT protein availability. Individuals with the 9-repeat/10-repeat genotype had a mean 22% reduction of DAT protein availability in putamen compared with 10-repeat homozygous individuals (t = 2.14, df = 23, p < .05). Consistent with earlier studies, alcoholism, per se, was not significantly associated with either DAT availability or DAT genotype. These findings suggest that the VNTR polymorphism of the DAT gene effects translation of the DAT protein. This effect may explain a variety of clinical associations that have been reported with this polymorphism. Copyright (C) 2000 American College of Neuropsychopharmacology.

Original language	English (US)
Pages (from-to)	133-139
Number of pages	7
Journal	Neuropsychopharmacology
Volume	22
Issue number	2
DOIs	https://doi.org/10.1016/S0893-133X(99)00099-8
State	Published - Feb 2000
Externally published	Yes

Keywords

Alcoholism
Dopamine transporter
Gene expression
SLC6A3
SPECT
β-CIT

ASJC Scopus subject areas

Pharmacology
Psychiatry and Mental health

Access to Document

10.1016/S0893-133X(99)00099-8

Cite this

@article{d63d01863f2c417dba3d27a99ecbfcc6,

title = "Genotype influences in vivo dopamine transporter availability in human striatum",

abstract = "In vivo availability of striatal dopamine transporter (DAT) protein has been reported to be reduced among alcoholics, and allelic variation of the DAT gene (SLC6A3) has been associated with severity of alcohol withdrawal. We examined the VNTR polymorphism of the 3' untranslated region of SLC6A3 and DAT protein availability in 14 abstinent alcoholics and 11 control subjects. Single photon emission computed tomography (SPECT) and plasma levels of the radioligand [I-123]β-CIT were used to quantify DAT protein availability. Individuals with the 9-repeat/10-repeat genotype had a mean 22% reduction of DAT protein availability in putamen compared with 10-repeat homozygous individuals (t = 2.14, df = 23, p < .05). Consistent with earlier studies, alcoholism, per se, was not significantly associated with either DAT availability or DAT genotype. These findings suggest that the VNTR polymorphism of the DAT gene effects translation of the DAT protein. This effect may explain a variety of clinical associations that have been reported with this polymorphism. Copyright (C) 2000 American College of Neuropsychopharmacology.",

keywords = "Alcoholism, Dopamine transporter, Gene expression, SLC6A3, SPECT, β-CIT",

author = "Andreas Heinz and David Goldman and Jones, {Douglas W.} and Roberta Palmour and Dan Hommer and Gorey, {Julia G.} and Lee, {Kan S.} and Markku Linnoila and Weinberger, {Daniel R.}",

note = "Funding Information: A. Heinz is supported in part by the Deutsche Forschungsge-meinschaft (Az: He 2597/1–1)",

year = "2000",

month = feb,

doi = "10.1016/S0893-133X(99)00099-8",

language = "English (US)",

volume = "22",

pages = "133--139",

journal = "Neuropsychopharmacology",

issn = "0893-133X",

publisher = "Nature Publishing Group",

number = "2",

}

TY - JOUR

T1 - Genotype influences in vivo dopamine transporter availability in human striatum

AU - Heinz, Andreas

AU - Goldman, David

AU - Jones, Douglas W.

AU - Palmour, Roberta

AU - Hommer, Dan

AU - Gorey, Julia G.

AU - Lee, Kan S.

AU - Linnoila, Markku

AU - Weinberger, Daniel R.

N1 - Funding Information: A. Heinz is supported in part by the Deutsche Forschungsge-meinschaft (Az: He 2597/1–1)

PY - 2000/2

Y1 - 2000/2

N2 - In vivo availability of striatal dopamine transporter (DAT) protein has been reported to be reduced among alcoholics, and allelic variation of the DAT gene (SLC6A3) has been associated with severity of alcohol withdrawal. We examined the VNTR polymorphism of the 3' untranslated region of SLC6A3 and DAT protein availability in 14 abstinent alcoholics and 11 control subjects. Single photon emission computed tomography (SPECT) and plasma levels of the radioligand [I-123]β-CIT were used to quantify DAT protein availability. Individuals with the 9-repeat/10-repeat genotype had a mean 22% reduction of DAT protein availability in putamen compared with 10-repeat homozygous individuals (t = 2.14, df = 23, p < .05). Consistent with earlier studies, alcoholism, per se, was not significantly associated with either DAT availability or DAT genotype. These findings suggest that the VNTR polymorphism of the DAT gene effects translation of the DAT protein. This effect may explain a variety of clinical associations that have been reported with this polymorphism. Copyright (C) 2000 American College of Neuropsychopharmacology.

AB - In vivo availability of striatal dopamine transporter (DAT) protein has been reported to be reduced among alcoholics, and allelic variation of the DAT gene (SLC6A3) has been associated with severity of alcohol withdrawal. We examined the VNTR polymorphism of the 3' untranslated region of SLC6A3 and DAT protein availability in 14 abstinent alcoholics and 11 control subjects. Single photon emission computed tomography (SPECT) and plasma levels of the radioligand [I-123]β-CIT were used to quantify DAT protein availability. Individuals with the 9-repeat/10-repeat genotype had a mean 22% reduction of DAT protein availability in putamen compared with 10-repeat homozygous individuals (t = 2.14, df = 23, p < .05). Consistent with earlier studies, alcoholism, per se, was not significantly associated with either DAT availability or DAT genotype. These findings suggest that the VNTR polymorphism of the DAT gene effects translation of the DAT protein. This effect may explain a variety of clinical associations that have been reported with this polymorphism. Copyright (C) 2000 American College of Neuropsychopharmacology.

KW - Alcoholism

KW - Dopamine transporter

KW - Gene expression

KW - SLC6A3

KW - SPECT

KW - β-CIT

UR - http://www.scopus.com/inward/record.url?scp=0034142232&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034142232&partnerID=8YFLogxK

U2 - 10.1016/S0893-133X(99)00099-8

DO - 10.1016/S0893-133X(99)00099-8

M3 - Article

C2 - 10649826

AN - SCOPUS:0034142232

SN - 0893-133X

VL - 22

SP - 133

EP - 139

JO - Neuropsychopharmacology

JF - Neuropsychopharmacology

IS - 2

ER -

Genotype influences in vivo dopamine transporter availability in human striatum

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this