Abstract
Analysis of genotypes for genetic predisposition of diseases has profound and widespread clinical application and has become a fundamental tool in the study of molecular epidemiology. In this study, we report a convenient and completely noninvasive approach to perform genotyping using terminal cephalic hair shaft. The average and median DNA amounts purified from the hair shaft are 112 ± 180 (1 SD) pg/mg and 66 pg/mg of hair shaft, respectively. Using hybridization with molecular beacons that can reliably distinguish single nucleotide polymorphisms at different alleles, we are able to analyze the genotypes of APO-E and G-protein β3 (GNB3) subunit C825T loci in DNA samples obtained from hair shaft in a high throughput format. The amount of DNA obtained from 20 mg of hair shaft is sufficient for multiple independent genotyping assays. These findings demonstrate that terminal hair shaft provides a convenient and reliable genomic DNA source for an analysis of genotypes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 925-929 |
| Number of pages | 5 |
| Journal | Cancer Epidemiology Biomarkers and Prevention |
| Volume | 11 |
| Issue number | 9 |
| State | Published - Sep 1 2002 |
ASJC Scopus subject areas
- Epidemiology
- Oncology