Genomics and the evolution of aminoacyl-tRNA synthesis

Benfang Ruan, Ivan Ahel, Alex Ambrogelly, Hubert D. Becker, Shipra Bunjun, Liang Feng, Debra Tumbula-Hansen, Michael Ibba, Dragana Korencic, Hiroyuki Kobayashi, Clarisse Jacquin-Becker, Nina Mejlhede, Bokkee Min, Gregory Raczniak, Jesse Rinehart, Constantinos Stathopoulos, Tong Li, Dieter Söll

Research output: Contribution to journalReview article

Abstract

Translation is the process by which ribosomes direct protein synthesis using the genetic information contained in messenger RNA (mRNA). Transfer RNAs (tRNAs) are charged with an amino acid and brought to the ribosome, where they are paired with the corresponding trinucleotide codon in mRNA. The amino acid is attached to the nascent polypeptide and the ribosome moves on to the next codon. Thus, the sequential pairing of codons in mRNA with tRNA anticodons determines the order of amino acids in a protein. It is therefore imperative for accurate translation that tRNAs are only coupled to amino acids corresponding to the RNA anticodon. This is mostly, but not exclusively, achieved by the direct attachment of the appropriate amino acid to the 3′-end of the corresponding tRNA by the aminoacyl-tRNA synthetases. To ensure the accurate translation of genetic information, the aminoacyl-tRNA synthetases must display an extremely high level of substrate specificity. Despite this highly conserved function, recent studies arising from the analysis of whole genomes have shown a significant degree of evolutionary diversity in aminoacyl-tRNA synthesis. For example, non-canonical routes have been identified for the synthesis of Asn-tRNA, Cys-tRNA, Gln-tRNA and Lys-tRNA. Characterization of non-canonical aminoacyl-tRNA synthesis has revealed an unexpected level of evolutionary divergence and has also provided new insights into the possible precursors of contemporary aminoacyl-tRNA synthetases.

Original languageEnglish (US)
Pages (from-to)313-321
Number of pages9
JournalActa biochimica Polonica
Volume48
Issue number2
DOIs
StatePublished - 2001

Keywords

  • Aminoacyl-tRNA
  • Evolution
  • Protein synthesis
  • Translation
  • tRNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Ruan, B., Ahel, I., Ambrogelly, A., Becker, H. D., Bunjun, S., Feng, L., Tumbula-Hansen, D., Ibba, M., Korencic, D., Kobayashi, H., Jacquin-Becker, C., Mejlhede, N., Min, B., Raczniak, G., Rinehart, J., Stathopoulos, C., Li, T., & Söll, D. (2001). Genomics and the evolution of aminoacyl-tRNA synthesis. Acta biochimica Polonica, 48(2), 313-321. https://doi.org/10.18388/abp.2001_3917