Abstract
p4O-phox is a newly isolated cytosolic component of the nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase that copurifies with p67- phox. Although its function is not well defined, preliminary evidence indicates that it is a component of the cytosolic complex. We report the characterization of the human p40-phox gene, which is single copy and spans approximately 18 kb with 10 exons. Based on fluorescent in situ hybridization (FISH) studies and analysis of somatic hybrid cell lines, the chromosomal location of p40-phox is human chromosome 22q13.1. The start of transcription has been mapped to bp -156. The expression of p4O-phox message is restricted to hematopoietic cells. In addition to identifying the mRNA transcript on Northern blot analysis in cells known to express components of the NADPH- oxidase, polymorphonuclear leukocytes, monocytes, B lymphoblastoid cell lines, and eosinophils, p4O-phox is also expressed in two other cell types of white cell lineage, mast cells, and basophils. In addition, the mRNA for p40- phox is expressed in megakaryocytic cells, but not in erythroid cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2714-2721 |
| Number of pages | 8 |
| Journal | Blood |
| Volume | 88 |
| Issue number | 7 |
| State | Published - Oct 1 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Hematology
Cite this
Genomic structure, chromosomal localization, start of transcription, and tissue expression of the human p40-phox, a new component of the nicotinamide adenine dinucleotide phosphate-oxidase complex. / Zhan, Shixing; Vazquez, Nancy; Zhan, Shili; Wientjes, Frans B.; Budarf, Marcia L.; Schrock, Evelin; Ried, Thomas; Green, Eric D.; Chanock, Stephen J.
In: Blood, Vol. 88, No. 7, 01.10.1996, p. 2714-2721.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genomic structure, chromosomal localization, start of transcription, and tissue expression of the human p40-phox, a new component of the nicotinamide adenine dinucleotide phosphate-oxidase complex
AU - Zhan, Shixing
AU - Vazquez, Nancy
AU - Zhan, Shili
AU - Wientjes, Frans B.
AU - Budarf, Marcia L.
AU - Schrock, Evelin
AU - Ried, Thomas
AU - Green, Eric D.
AU - Chanock, Stephen J.
PY - 1996/10/1
Y1 - 1996/10/1
N2 - p4O-phox is a newly isolated cytosolic component of the nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase that copurifies with p67- phox. Although its function is not well defined, preliminary evidence indicates that it is a component of the cytosolic complex. We report the characterization of the human p40-phox gene, which is single copy and spans approximately 18 kb with 10 exons. Based on fluorescent in situ hybridization (FISH) studies and analysis of somatic hybrid cell lines, the chromosomal location of p40-phox is human chromosome 22q13.1. The start of transcription has been mapped to bp -156. The expression of p4O-phox message is restricted to hematopoietic cells. In addition to identifying the mRNA transcript on Northern blot analysis in cells known to express components of the NADPH- oxidase, polymorphonuclear leukocytes, monocytes, B lymphoblastoid cell lines, and eosinophils, p4O-phox is also expressed in two other cell types of white cell lineage, mast cells, and basophils. In addition, the mRNA for p40- phox is expressed in megakaryocytic cells, but not in erythroid cells.
AB - p4O-phox is a newly isolated cytosolic component of the nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase that copurifies with p67- phox. Although its function is not well defined, preliminary evidence indicates that it is a component of the cytosolic complex. We report the characterization of the human p40-phox gene, which is single copy and spans approximately 18 kb with 10 exons. Based on fluorescent in situ hybridization (FISH) studies and analysis of somatic hybrid cell lines, the chromosomal location of p40-phox is human chromosome 22q13.1. The start of transcription has been mapped to bp -156. The expression of p4O-phox message is restricted to hematopoietic cells. In addition to identifying the mRNA transcript on Northern blot analysis in cells known to express components of the NADPH- oxidase, polymorphonuclear leukocytes, monocytes, B lymphoblastoid cell lines, and eosinophils, p4O-phox is also expressed in two other cell types of white cell lineage, mast cells, and basophils. In addition, the mRNA for p40- phox is expressed in megakaryocytic cells, but not in erythroid cells.
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M3 - Article
VL - 88
SP - 2714
EP - 2721
JO - Blood
JF - Blood
SN - 0006-4971
IS - 7
ER -