Genomic structure, chromosomal localization, start of transcription, and tissue expression of the human p40-phox, a new component of the nicotinamide adenine dinucleotide phosphate-oxidase complex

Shixing Zhan, Nancy Vazquez, Shili Zhan, Frans B. Wientjes, Marcia L. Budarf, Evelin Schrock, Thomas Ried, Eric D. Green, Stephen J. Chanock

Research output: Contribution to journalArticlepeer-review

Abstract

p4O-phox is a newly isolated cytosolic component of the nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase that copurifies with p67- phox. Although its function is not well defined, preliminary evidence indicates that it is a component of the cytosolic complex. We report the characterization of the human p40-phox gene, which is single copy and spans approximately 18 kb with 10 exons. Based on fluorescent in situ hybridization (FISH) studies and analysis of somatic hybrid cell lines, the chromosomal location of p40-phox is human chromosome 22q13.1. The start of transcription has been mapped to bp -156. The expression of p4O-phox message is restricted to hematopoietic cells. In addition to identifying the mRNA transcript on Northern blot analysis in cells known to express components of the NADPH- oxidase, polymorphonuclear leukocytes, monocytes, B lymphoblastoid cell lines, and eosinophils, p4O-phox is also expressed in two other cell types of white cell lineage, mast cells, and basophils. In addition, the mRNA for p40- phox is expressed in megakaryocytic cells, but not in erythroid cells.

Original languageEnglish (US)
Pages (from-to)2714-2721
Number of pages8
JournalBlood
Volume88
Issue number7
DOIs
StatePublished - Oct 1 1996

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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