Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi

Claire M. Fraser; Sherwood Casjens; Wai Mun Huang; Granger G. Sutton; Rebecca Clayton; Raju Lathigra; Owen White; Karen A. Ketchum; Robert Dodson; Erin K. Hickey; Michelle Gwinn; Brian Dougherty; Jean Francois Tomb; Robert D. Fleischmann; Delwood Richardson; Jeremy Peterson; Anthony R. Kerlavage; John Quackenbush; Steven Salzberg; Mark Hanson; Rene Van Vugt; Nanette Palmer; Mark D. Adams; Jeannine Gocayne; Janice Weidman; Teresa Utterback; Larry Watthey; Lisa McDonald; Patricia Artiach; Cheryl Bowman; Stacey Garland; Claire Fujii; Matthew D. Cotton; Kurt Horst; Kevin Roberts; Bonnie Hatch; Hamilton O. Smith; J. Craig Venter

doi:10.1038/37551

Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi

Claire M. Fraser, Sherwood Casjens, Wai Mun Huang, Granger G. Sutton, Rebecca Clayton, Raju Lathigra, Owen White, Karen A. Ketchum, Robert Dodson, Erin K. Hickey, Michelle Gwinn, Brian Dougherty, Jean Francois Tomb, Robert D. Fleischmann, Delwood Richardson, Jeremy Peterson, Anthony R. Kerlavage, John Quackenbush, Steven Salzberg, Mark HansonRene Van Vugt, Nanette Palmer, Mark D. Adams, Jeannine Gocayne, Janice Weidman, Teresa Utterback, Larry Watthey, Lisa McDonald, Patricia Artiach, Cheryl Bowman, Stacey Garland, Claire Fujii, Matthew D. Cotton, Kurt Horst, Kevin Roberts, Bonnie Hatch, Hamilton O. Smith, J. Craig Venter

Research output: Contribution to journal › Article › peer-review

1606 Scopus citations

Abstract

The genome of the bacterium Borrelia burgdorferi B31, the aetiologic agent of Lyme disease, contains a linear chromosome of 910, 725 base pairs and at least 17 linear and circular plasmids with a combined size of more than 533, 000 base pairs. The chromosome contains 853 genes encoding a basic set of proteins for DNA replication, transcription, translation, solute transport and energy metabolism, but, like Mycoplasma genitallum, it contains no genes for cellular biosynthetic reactions. Because B. burgdorferi and M. genitalium are distantly related eubacteria, we suggest that their limited metabolic capacities reflect convergent evolution by gene loss from more metabolically competent progenitors. Of 430 genes on 11 plasmids, most have no known biological function; 39% of plasmid genes are paralogues that form 47 gene families. The biological significance of the multiple plasmid- encoded genes is not clear, although they may be involved in antigenic variation or immune evasion.

Original language	English (US)
Pages (from-to)	580-586
Number of pages	7
Journal	Nature
Volume	390
Issue number	6660
DOIs	https://doi.org/10.1038/37551
State	Published - Dec 11 1997
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1038/37551

Cite this

Fraser, C. M., Casjens, S., Huang, W. M., Sutton, G. G., Clayton, R., Lathigra, R., White, O., Ketchum, K. A., Dodson, R., Hickey, E. K., Gwinn, M., Dougherty, B., Tomb, J. F., Fleischmann, R. D., Richardson, D., Peterson, J., Kerlavage, A. R., Quackenbush, J., Salzberg, S., ... Venter, J. C. (1997). Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi. Nature, 390(6660), 580-586. https://doi.org/10.1038/37551

Fraser, CM, Casjens, S, Huang, WM, Sutton, GG, Clayton, R, Lathigra, R, White, O, Ketchum, KA, Dodson, R, Hickey, EK, Gwinn, M, Dougherty, B, Tomb, JF, Fleischmann, RD, Richardson, D, Peterson, J, Kerlavage, AR, Quackenbush, J, Salzberg, S, Hanson, M, Van Vugt, R, Palmer, N, Adams, MD, Gocayne, J, Weidman, J, Utterback, T, Watthey, L, McDonald, L, Artiach, P, Bowman, C, Garland, S, Fujii, C, Cotton, MD, Horst, K, Roberts, K, Hatch, B, Smith, HO & Venter, JC 1997, 'Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi', Nature, vol. 390, no. 6660, pp. 580-586. https://doi.org/10.1038/37551

@article{f3c16493233a40769614c65c8c28d9ac,

title = "Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi",

abstract = "The genome of the bacterium Borrelia burgdorferi B31, the aetiologic agent of Lyme disease, contains a linear chromosome of 910, 725 base pairs and at least 17 linear and circular plasmids with a combined size of more than 533, 000 base pairs. The chromosome contains 853 genes encoding a basic set of proteins for DNA replication, transcription, translation, solute transport and energy metabolism, but, like Mycoplasma genitallum, it contains no genes for cellular biosynthetic reactions. Because B. burgdorferi and M. genitalium are distantly related eubacteria, we suggest that their limited metabolic capacities reflect convergent evolution by gene loss from more metabolically competent progenitors. Of 430 genes on 11 plasmids, most have no known biological function; 39% of plasmid genes are paralogues that form 47 gene families. The biological significance of the multiple plasmid- encoded genes is not clear, although they may be involved in antigenic variation or immune evasion.",

author = "Fraser, {Claire M.} and Sherwood Casjens and Huang, {Wai Mun} and Sutton, {Granger G.} and Rebecca Clayton and Raju Lathigra and Owen White and Ketchum, {Karen A.} and Robert Dodson and Hickey, {Erin K.} and Michelle Gwinn and Brian Dougherty and Tomb, {Jean Francois} and Fleischmann, {Robert D.} and Delwood Richardson and Jeremy Peterson and Kerlavage, {Anthony R.} and John Quackenbush and Steven Salzberg and Mark Hanson and {Van Vugt}, Rene and Nanette Palmer and Adams, {Mark D.} and Jeannine Gocayne and Janice Weidman and Teresa Utterback and Larry Watthey and Lisa McDonald and Patricia Artiach and Cheryl Bowman and Stacey Garland and Claire Fujii and Cotton, {Matthew D.} and Kurt Horst and Kevin Roberts and Bonnie Hatch and Smith, {Hamilton O.} and Venter, {J. Craig}",

year = "1997",

month = dec,

day = "11",

doi = "10.1038/37551",

language = "English (US)",

volume = "390",

pages = "580--586",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Publishing Group",

number = "6660",

}

TY - JOUR

T1 - Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi

AU - Fraser, Claire M.

AU - Casjens, Sherwood

AU - Huang, Wai Mun

AU - Sutton, Granger G.

AU - Clayton, Rebecca

AU - Lathigra, Raju

AU - White, Owen

AU - Ketchum, Karen A.

AU - Dodson, Robert

AU - Hickey, Erin K.

AU - Gwinn, Michelle

AU - Dougherty, Brian

AU - Tomb, Jean Francois

AU - Fleischmann, Robert D.

AU - Richardson, Delwood

AU - Peterson, Jeremy

AU - Kerlavage, Anthony R.

AU - Quackenbush, John

AU - Salzberg, Steven

AU - Hanson, Mark

AU - Van Vugt, Rene

AU - Palmer, Nanette

AU - Adams, Mark D.

AU - Gocayne, Jeannine

AU - Weidman, Janice

AU - Utterback, Teresa

AU - Watthey, Larry

AU - McDonald, Lisa

AU - Artiach, Patricia

AU - Bowman, Cheryl

AU - Garland, Stacey

AU - Fujii, Claire

AU - Cotton, Matthew D.

AU - Horst, Kurt

AU - Roberts, Kevin

AU - Hatch, Bonnie

AU - Smith, Hamilton O.

AU - Venter, J. Craig

PY - 1997/12/11

Y1 - 1997/12/11

N2 - The genome of the bacterium Borrelia burgdorferi B31, the aetiologic agent of Lyme disease, contains a linear chromosome of 910, 725 base pairs and at least 17 linear and circular plasmids with a combined size of more than 533, 000 base pairs. The chromosome contains 853 genes encoding a basic set of proteins for DNA replication, transcription, translation, solute transport and energy metabolism, but, like Mycoplasma genitallum, it contains no genes for cellular biosynthetic reactions. Because B. burgdorferi and M. genitalium are distantly related eubacteria, we suggest that their limited metabolic capacities reflect convergent evolution by gene loss from more metabolically competent progenitors. Of 430 genes on 11 plasmids, most have no known biological function; 39% of plasmid genes are paralogues that form 47 gene families. The biological significance of the multiple plasmid- encoded genes is not clear, although they may be involved in antigenic variation or immune evasion.

AB - The genome of the bacterium Borrelia burgdorferi B31, the aetiologic agent of Lyme disease, contains a linear chromosome of 910, 725 base pairs and at least 17 linear and circular plasmids with a combined size of more than 533, 000 base pairs. The chromosome contains 853 genes encoding a basic set of proteins for DNA replication, transcription, translation, solute transport and energy metabolism, but, like Mycoplasma genitallum, it contains no genes for cellular biosynthetic reactions. Because B. burgdorferi and M. genitalium are distantly related eubacteria, we suggest that their limited metabolic capacities reflect convergent evolution by gene loss from more metabolically competent progenitors. Of 430 genes on 11 plasmids, most have no known biological function; 39% of plasmid genes are paralogues that form 47 gene families. The biological significance of the multiple plasmid- encoded genes is not clear, although they may be involved in antigenic variation or immune evasion.

UR - http://www.scopus.com/inward/record.url?scp=0031470826&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031470826&partnerID=8YFLogxK

U2 - 10.1038/37551

DO - 10.1038/37551

M3 - Article

C2 - 9403685

AN - SCOPUS:0031470826

SN - 0028-0836

VL - 390

SP - 580

EP - 586

JO - Nature

JF - Nature

IS - 6660

ER -

Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this