TY - JOUR
T1 - Genomic organization of the human PMS2 gene family
AU - Nicolaides, Nicholas C.
AU - Carter, Kenneth C.
AU - Shell, Brenda K.
AU - Papadopoulos, Nickolas
AU - Vogelstein, Bert
AU - Kinzler, Kenneth W.
N1 - Funding Information:
We thank Drs. S. W. Scherer and L.-C. Tsui (University of Toronto) for somatic cell hybrids. We thank Drs. C. Lengauer and G. Riggins for critical readings of the manuscript. This work was supported by NIH Grants CA35494, CA62924, and CA57345. B.V. is an American Cancer Society Research Professor and an investigator with the Howard Hughes Medical Institute.
PY - 1995/11
Y1 - 1995/11
N2 - ThehPMS2gene (HGMW-approved symbolPMS2) encodes amutLhomolog that causes hereditary nonpolyposis colon cancer (HNPCC) when inherited in mutant form. We have here characterized the genomic structure of thehPMS2gene to facilitate its analysis in HNPCC kindreds. ThehPMS2genomic locus was found to encompass 16 kb and consist of 15 exons. During its analysis, we identified a family ofhPMS2-related genes located on chromosome 7 at bands 7p12-p13, 7q11, and 7q22. Exons 1 through 5 of these homologs shared a high degree of identity withhPMS2.We present the sequence of seven novel genes that represent thehPMSR(hPMS2-related) gene family. The similarity and number of these genes made specific amplification ofhPMS2problematic, but knowledge of them aided the successful design of oligonucleotides for this purpose.
AB - ThehPMS2gene (HGMW-approved symbolPMS2) encodes amutLhomolog that causes hereditary nonpolyposis colon cancer (HNPCC) when inherited in mutant form. We have here characterized the genomic structure of thehPMS2gene to facilitate its analysis in HNPCC kindreds. ThehPMS2genomic locus was found to encompass 16 kb and consist of 15 exons. During its analysis, we identified a family ofhPMS2-related genes located on chromosome 7 at bands 7p12-p13, 7q11, and 7q22. Exons 1 through 5 of these homologs shared a high degree of identity withhPMS2.We present the sequence of seven novel genes that represent thehPMSR(hPMS2-related) gene family. The similarity and number of these genes made specific amplification ofhPMS2problematic, but knowledge of them aided the successful design of oligonucleotides for this purpose.
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U2 - 10.1006/geno.1995.9885
DO - 10.1006/geno.1995.9885
M3 - Article
C2 - 8586419
AN - SCOPUS:0028849441
SN - 0888-7543
VL - 30
SP - 195
EP - 206
JO - Genomics
JF - Genomics
IS - 2
ER -