Genomic organization and sequences of immunoglobulin light chain genes in a primitive vertebrate suggest coevolution of immunoglobulin gene organization

M. J. Shamblott, G. W. Litman

Research output: Contribution to journalArticle

Abstract

The genomic organization and sequence of immunoglobulin light chain genes in Heterodontus francisci (horned shark), a phylogenetically primitive vertebrate, have been characterized. Light chain variable (V(L)) and joining (J(L)) segments are separated by 380 nucleotides and together with the single constant region exon (C(L)), occupy <2.7 kb, the closest linkage described thus far for a rearranging gene system. The V(L) segment is flanked by a characteristic recombination signal sequence possessing a 12 nucleotide spacer; the recombination signal sequence flanking the J(L) segment is 23 nucleotides. The V(L) genes, unlike heavy chain genes, possess a typical upstream regulatory octamer as well as conserved enhancer core sequences in the intervening sequence separating J(L) and C(L). Restriction mapping and genomic Southern blotting are consistent with the presence of multiple light chain gene clusters. There appear to be considerably fewer light than heavy chain genes. Heavy and light chain clusters show no evidence of genomic linkage using field inversion gel electrophoresis. The findings of major differences in the organization and functional rearrangement properties of immunoglobulin genes in species representing different levels of vertebrate evolution, but consistent similarity in the organization of heavy and light chain genes within a species, suggests that these systems may be coevolving.

Original languageEnglish (US)
Pages (from-to)3733-3739
Number of pages7
JournalThe EMBO journal
Volume8
Issue number12
StatePublished - 1989
Externally publishedYes

Fingerprint

Immunoglobulin Light Chain Genes
Immunoglobulin Light Chains
Immunoglobulin Genes
Vertebrates
Immunoglobulins
Genes
Light
Nucleotides
Protein Sorting Signals
Genetic Recombination
Sharks
Restriction Mapping
Pulsed Field Gel Electrophoresis
Multigene Family
Southern Blotting
Introns
Exons
Electrophoresis
Joining
Gels

Keywords

  • coevolution
  • field inversion gel electrophoresis
  • immunoglobulin
  • recombination signal sequence
  • regulatory octamer
  • V(L) gene organization

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Genomic organization and sequences of immunoglobulin light chain genes in a primitive vertebrate suggest coevolution of immunoglobulin gene organization. / Shamblott, M. J.; Litman, G. W.

In: The EMBO journal, Vol. 8, No. 12, 1989, p. 3733-3739.

Research output: Contribution to journalArticle

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