TY - JOUR
T1 - Genomic diversity of SARS-CoV-2 during early introduction into the Baltimore-Washington metropolitan area
AU - Thielen, Peter M.
AU - Wohl, Shirlee
AU - Mehoke, Thomas
AU - Ramakrishnan, Srividya
AU - Kirsche, Melanie
AU - Falade-Nwulia, Oluwaseun
AU - Trovão, Nídia S.
AU - Ernlund, Amanda
AU - Howser, Craig
AU - Sadowski, Norah
AU - Morris, C. Paul
AU - Hopkins, Mark
AU - Schwartz, Matthew
AU - Fan, Yunfan
AU - Gniazdowski, Victoria
AU - Lessler, Justin
AU - Sauer, Lauren
AU - Schatz, Michael C.
AU - Evans, Jared D.
AU - Ray, Stuart C.
AU - Timp, Winston
AU - Mostafa, Heba H.
N1 - Publisher Copyright:
© 2021, Thielen et al. This is an open access article published under the terms of the Creative Commons Attribution 4.0 International License.
PY - 2021/3/22
Y1 - 2021/3/22
N2 - The early COVID-19 pandemic was characterized by rapid global spread. In Maryland and Washington, DC, United States, more than 2500 cases were reported within 3 weeks of the first COVID-19 detection in March 2020. We aimed to use genomic sequencing to understand the initial spread of SARS-CoV-2 - the virus that causes COVID-19 - in the region. We analyzed 620 samples collected from the Johns Hopkins Health System during March 11-31, 2020, comprising 28.6% of the total cases in Maryland and Washington, DC. From these samples, we generated 114 complete viral genomes. Analysis of these genomes alongside a subsampling of over 1000 previously published sequences showed that the diversity in this region rivaled global SARS-CoV-2 genetic diversity at that time and that the sequences belong to all of the major globally circulating lineages, suggesting multiple introductions into the region. We also analyzed these regional SARS-CoV-2 genomes alongside detailed clinical metadata and found that clinically severe cases had viral genomes belonging to all major viral lineages. We conclude that efforts to control local spread of the virus were likely confounded by the number of introductions into the region early in the epidemic and the interconnectedness of the region as a whole.
AB - The early COVID-19 pandemic was characterized by rapid global spread. In Maryland and Washington, DC, United States, more than 2500 cases were reported within 3 weeks of the first COVID-19 detection in March 2020. We aimed to use genomic sequencing to understand the initial spread of SARS-CoV-2 - the virus that causes COVID-19 - in the region. We analyzed 620 samples collected from the Johns Hopkins Health System during March 11-31, 2020, comprising 28.6% of the total cases in Maryland and Washington, DC. From these samples, we generated 114 complete viral genomes. Analysis of these genomes alongside a subsampling of over 1000 previously published sequences showed that the diversity in this region rivaled global SARS-CoV-2 genetic diversity at that time and that the sequences belong to all of the major globally circulating lineages, suggesting multiple introductions into the region. We also analyzed these regional SARS-CoV-2 genomes alongside detailed clinical metadata and found that clinically severe cases had viral genomes belonging to all major viral lineages. We conclude that efforts to control local spread of the virus were likely confounded by the number of introductions into the region early in the epidemic and the interconnectedness of the region as a whole.
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U2 - 10.1172/jci.insight.144350
DO - 10.1172/jci.insight.144350
M3 - Article
C2 - 33749660
AN - SCOPUS:85103277349
SN - 2379-3708
VL - 6
JO - JCI Insight
JF - JCI Insight
IS - 6
M1 - e144350
ER -