Genome-Wide Mapping of Yeast RNA Polymerase II Termination

Paul Schaughency, Jonathan Merran, Jeffry Lynn Corden

Research output: Contribution to journalArticle

Abstract

Yeast RNA polymerase II (Pol II) terminates transcription of coding transcripts through the polyadenylation (pA) pathway and non-coding transcripts through the non-polyadenylation (non-pA) pathway. We have used PAR-CLIP to map the position of Pol II genome-wide in living yeast cells after depletion of components of either the pA or non-pA termination complexes. We show here that Ysh1, responsible for cleavage at the pA site, is required for efficient removal of Pol II from the template. Depletion of Ysh1 from the nucleus does not, however, lead to readthrough transcription. In contrast, depletion of the termination factor Nrd1 leads to widespread runaway elongation of non-pA transcripts. Depletion of Sen1 also leads to readthrough at non-pA terminators, but in contrast to Nrd1, this readthrough is less processive, or more susceptible to pausing. The data presented here provide delineation of in vivo Pol II termination regions and highlight differences in the sequences that signal termination of different classes of non-pA transcripts.

Original languageEnglish (US)
JournalPLoS Genetics
Volume10
Issue number10
DOIs
StatePublished - Oct 1 2014

Fingerprint

Polyadenylation
RNA Polymerase II
Chromosome Mapping
DNA-directed RNA polymerase
yeast
RNA
genome
transcription (genetics)
Yeasts
yeasts
photosynthetically active radiation
signal peptide
cleavage
Homeless Youth
Protein Sorting Signals
Genome
cells
removal

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

Genome-Wide Mapping of Yeast RNA Polymerase II Termination. / Schaughency, Paul; Merran, Jonathan; Corden, Jeffry Lynn.

In: PLoS Genetics, Vol. 10, No. 10, 01.10.2014.

Research output: Contribution to journalArticle

Schaughency, Paul ; Merran, Jonathan ; Corden, Jeffry Lynn. / Genome-Wide Mapping of Yeast RNA Polymerase II Termination. In: PLoS Genetics. 2014 ; Vol. 10, No. 10.
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