Genome-wide Mapping of HATs and HDACs Reveals Distinct Functions in Active and Inactive Genes

Zhibin Wang, Chongzhi Zang, Kairong Cui, Dustin E. Schones, Artem Barski, Weiqun Peng, Keji Zhao

Research output: Contribution to journalArticle

Abstract

Histone acetyltransferases (HATs) and deacetylases (HDACs) function antagonistically to control histone acetylation. As acetylation is a histone mark for active transcription, HATs have been associated with active and HDACs with inactive genes. We describe here genome-wide mapping of HATs and HDACs binding on chromatin and find that both are found at active genes with acetylated histones. Our data provide evidence that HATs and HDACs are both targeted to transcribed regions of active genes by phosphorylated RNA Pol II. Furthermore, the majority of HDACs in the human genome function to reset chromatin by removing acetylation at active genes. Inactive genes that are primed by MLL-mediated histone H3K4 methylation are subject to a dynamic cycle of acetylation and deacetylation by transient HAT/HDAC binding, preventing Pol II from binding to these genes but poising them for future activation. Silent genes without any H3K4 methylation signal show no evidence of being bound by HDACs.

Original languageEnglish (US)
Pages (from-to)1019-1031
Number of pages13
JournalCell
Volume138
Issue number5
DOIs
StatePublished - Sep 4 2009
Externally publishedYes

Fingerprint

Histone Acetyltransferases
Histone Deacetylases
Chromosome Mapping
Genes
Acetylation
Histones
Methylation
Chromatin
Histone Code
RNA Polymerase II
Human Genome
Transcription
Chemical activation

Keywords

  • DNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Wang, Z., Zang, C., Cui, K., Schones, D. E., Barski, A., Peng, W., & Zhao, K. (2009). Genome-wide Mapping of HATs and HDACs Reveals Distinct Functions in Active and Inactive Genes. Cell, 138(5), 1019-1031. https://doi.org/10.1016/j.cell.2009.06.049

Genome-wide Mapping of HATs and HDACs Reveals Distinct Functions in Active and Inactive Genes. / Wang, Zhibin; Zang, Chongzhi; Cui, Kairong; Schones, Dustin E.; Barski, Artem; Peng, Weiqun; Zhao, Keji.

In: Cell, Vol. 138, No. 5, 04.09.2009, p. 1019-1031.

Research output: Contribution to journalArticle

Wang, Z, Zang, C, Cui, K, Schones, DE, Barski, A, Peng, W & Zhao, K 2009, 'Genome-wide Mapping of HATs and HDACs Reveals Distinct Functions in Active and Inactive Genes', Cell, vol. 138, no. 5, pp. 1019-1031. https://doi.org/10.1016/j.cell.2009.06.049
Wang, Zhibin ; Zang, Chongzhi ; Cui, Kairong ; Schones, Dustin E. ; Barski, Artem ; Peng, Weiqun ; Zhao, Keji. / Genome-wide Mapping of HATs and HDACs Reveals Distinct Functions in Active and Inactive Genes. In: Cell. 2009 ; Vol. 138, No. 5. pp. 1019-1031.
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