Genome-wide functional analysis of pathogenicity genes in the rice blast fungus

Junhyun Jeon, Sook Young Park, Myoung Hwan Chi, Jaehyuk Choi, Jongsun Park, Hee Sool Rho, Soonok Kim, Jaeduk Goh, Sungyong Yoo, Jinhee Choi, Ju Young Park, Mihwa Yi, Seonyoung Yang, Min Jung Kwon, Seong Sook Han, Byeong Ryun Kim, Chang Hyun Khang, Bongsoo Park, Se Eun Lim, Kyongyong Jung & 10 others Sunghyung Kong, Maruthachalam Karunakaran, Hong Sik Oh, Hyojeong Kim, Seryun Kim, Jaejin Park, Soyoung Kang, Woo Bong Choi, Seogchan Kang, Yong Hwan Lee

Research output: Contribution to journalArticle

Abstract

Rapid translation of genome sequences into meaningful biological information hinges on the integration of multiple experimental and informatics methods into a cohesive platform. Despite the explosion in the number of genome sequences available, such a platform does not exist for filamentous fungi. Here we present the development and application of a functional genomics and informatics platform for a model plant pathogenic fungus, Magnaporthe oryzae. In total, we produced 21,070 mutants through large-scale insertional mutagenesis using Agrobacterium tumefaciens-mediated transformation. We used a high-throughput phenotype screening pipeline to detect disruption of seven phenotypes encompassing the fungal life cycle and identified the mutated gene and the nature of mutation for each mutant. Comparative analysis of phenotypes and genotypes of the mutants uncovered 202 new pathogenicity loci. Our findings demonstrate the effectiveness of our platform and provide new insights on the molecular basis of fungal pathogenesis. Our approach promises comprehensive functional genomics in filamentous fungi and beyond.

Original languageEnglish (US)
Pages (from-to)561-565
Number of pages5
JournalNature Genetics
Volume39
Issue number4
DOIs
StatePublished - Apr 2007
Externally publishedYes

Fingerprint

Virulence
Informatics
Fungi
Genome
Genomics
Phenotype
Magnaporthe
Genes
Agrobacterium tumefaciens
Explosions
Insertional Mutagenesis
Life Cycle Stages
Genotype
Mutation
Oryza

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Jeon, J., Park, S. Y., Chi, M. H., Choi, J., Park, J., Rho, H. S., ... Lee, Y. H. (2007). Genome-wide functional analysis of pathogenicity genes in the rice blast fungus. Nature Genetics, 39(4), 561-565. https://doi.org/10.1038/ng2002

Genome-wide functional analysis of pathogenicity genes in the rice blast fungus. / Jeon, Junhyun; Park, Sook Young; Chi, Myoung Hwan; Choi, Jaehyuk; Park, Jongsun; Rho, Hee Sool; Kim, Soonok; Goh, Jaeduk; Yoo, Sungyong; Choi, Jinhee; Park, Ju Young; Yi, Mihwa; Yang, Seonyoung; Kwon, Min Jung; Han, Seong Sook; Kim, Byeong Ryun; Khang, Chang Hyun; Park, Bongsoo; Lim, Se Eun; Jung, Kyongyong; Kong, Sunghyung; Karunakaran, Maruthachalam; Oh, Hong Sik; Kim, Hyojeong; Kim, Seryun; Park, Jaejin; Kang, Soyoung; Choi, Woo Bong; Kang, Seogchan; Lee, Yong Hwan.

In: Nature Genetics, Vol. 39, No. 4, 04.2007, p. 561-565.

Research output: Contribution to journalArticle

Jeon, J, Park, SY, Chi, MH, Choi, J, Park, J, Rho, HS, Kim, S, Goh, J, Yoo, S, Choi, J, Park, JY, Yi, M, Yang, S, Kwon, MJ, Han, SS, Kim, BR, Khang, CH, Park, B, Lim, SE, Jung, K, Kong, S, Karunakaran, M, Oh, HS, Kim, H, Kim, S, Park, J, Kang, S, Choi, WB, Kang, S & Lee, YH 2007, 'Genome-wide functional analysis of pathogenicity genes in the rice blast fungus', Nature Genetics, vol. 39, no. 4, pp. 561-565. https://doi.org/10.1038/ng2002
Jeon J, Park SY, Chi MH, Choi J, Park J, Rho HS et al. Genome-wide functional analysis of pathogenicity genes in the rice blast fungus. Nature Genetics. 2007 Apr;39(4):561-565. https://doi.org/10.1038/ng2002
Jeon, Junhyun ; Park, Sook Young ; Chi, Myoung Hwan ; Choi, Jaehyuk ; Park, Jongsun ; Rho, Hee Sool ; Kim, Soonok ; Goh, Jaeduk ; Yoo, Sungyong ; Choi, Jinhee ; Park, Ju Young ; Yi, Mihwa ; Yang, Seonyoung ; Kwon, Min Jung ; Han, Seong Sook ; Kim, Byeong Ryun ; Khang, Chang Hyun ; Park, Bongsoo ; Lim, Se Eun ; Jung, Kyongyong ; Kong, Sunghyung ; Karunakaran, Maruthachalam ; Oh, Hong Sik ; Kim, Hyojeong ; Kim, Seryun ; Park, Jaejin ; Kang, Soyoung ; Choi, Woo Bong ; Kang, Seogchan ; Lee, Yong Hwan. / Genome-wide functional analysis of pathogenicity genes in the rice blast fungus. In: Nature Genetics. 2007 ; Vol. 39, No. 4. pp. 561-565.
@article{52bd82d070654bca8713e21bcf01684f,
title = "Genome-wide functional analysis of pathogenicity genes in the rice blast fungus",
abstract = "Rapid translation of genome sequences into meaningful biological information hinges on the integration of multiple experimental and informatics methods into a cohesive platform. Despite the explosion in the number of genome sequences available, such a platform does not exist for filamentous fungi. Here we present the development and application of a functional genomics and informatics platform for a model plant pathogenic fungus, Magnaporthe oryzae. In total, we produced 21,070 mutants through large-scale insertional mutagenesis using Agrobacterium tumefaciens-mediated transformation. We used a high-throughput phenotype screening pipeline to detect disruption of seven phenotypes encompassing the fungal life cycle and identified the mutated gene and the nature of mutation for each mutant. Comparative analysis of phenotypes and genotypes of the mutants uncovered 202 new pathogenicity loci. Our findings demonstrate the effectiveness of our platform and provide new insights on the molecular basis of fungal pathogenesis. Our approach promises comprehensive functional genomics in filamentous fungi and beyond.",
author = "Junhyun Jeon and Park, {Sook Young} and Chi, {Myoung Hwan} and Jaehyuk Choi and Jongsun Park and Rho, {Hee Sool} and Soonok Kim and Jaeduk Goh and Sungyong Yoo and Jinhee Choi and Park, {Ju Young} and Mihwa Yi and Seonyoung Yang and Kwon, {Min Jung} and Han, {Seong Sook} and Kim, {Byeong Ryun} and Khang, {Chang Hyun} and Bongsoo Park and Lim, {Se Eun} and Kyongyong Jung and Sunghyung Kong and Maruthachalam Karunakaran and Oh, {Hong Sik} and Hyojeong Kim and Seryun Kim and Jaejin Park and Soyoung Kang and Choi, {Woo Bong} and Seogchan Kang and Lee, {Yong Hwan}",
year = "2007",
month = "4",
doi = "10.1038/ng2002",
language = "English (US)",
volume = "39",
pages = "561--565",
journal = "Nature Genetics",
issn = "1061-4036",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - Genome-wide functional analysis of pathogenicity genes in the rice blast fungus

AU - Jeon, Junhyun

AU - Park, Sook Young

AU - Chi, Myoung Hwan

AU - Choi, Jaehyuk

AU - Park, Jongsun

AU - Rho, Hee Sool

AU - Kim, Soonok

AU - Goh, Jaeduk

AU - Yoo, Sungyong

AU - Choi, Jinhee

AU - Park, Ju Young

AU - Yi, Mihwa

AU - Yang, Seonyoung

AU - Kwon, Min Jung

AU - Han, Seong Sook

AU - Kim, Byeong Ryun

AU - Khang, Chang Hyun

AU - Park, Bongsoo

AU - Lim, Se Eun

AU - Jung, Kyongyong

AU - Kong, Sunghyung

AU - Karunakaran, Maruthachalam

AU - Oh, Hong Sik

AU - Kim, Hyojeong

AU - Kim, Seryun

AU - Park, Jaejin

AU - Kang, Soyoung

AU - Choi, Woo Bong

AU - Kang, Seogchan

AU - Lee, Yong Hwan

PY - 2007/4

Y1 - 2007/4

N2 - Rapid translation of genome sequences into meaningful biological information hinges on the integration of multiple experimental and informatics methods into a cohesive platform. Despite the explosion in the number of genome sequences available, such a platform does not exist for filamentous fungi. Here we present the development and application of a functional genomics and informatics platform for a model plant pathogenic fungus, Magnaporthe oryzae. In total, we produced 21,070 mutants through large-scale insertional mutagenesis using Agrobacterium tumefaciens-mediated transformation. We used a high-throughput phenotype screening pipeline to detect disruption of seven phenotypes encompassing the fungal life cycle and identified the mutated gene and the nature of mutation for each mutant. Comparative analysis of phenotypes and genotypes of the mutants uncovered 202 new pathogenicity loci. Our findings demonstrate the effectiveness of our platform and provide new insights on the molecular basis of fungal pathogenesis. Our approach promises comprehensive functional genomics in filamentous fungi and beyond.

AB - Rapid translation of genome sequences into meaningful biological information hinges on the integration of multiple experimental and informatics methods into a cohesive platform. Despite the explosion in the number of genome sequences available, such a platform does not exist for filamentous fungi. Here we present the development and application of a functional genomics and informatics platform for a model plant pathogenic fungus, Magnaporthe oryzae. In total, we produced 21,070 mutants through large-scale insertional mutagenesis using Agrobacterium tumefaciens-mediated transformation. We used a high-throughput phenotype screening pipeline to detect disruption of seven phenotypes encompassing the fungal life cycle and identified the mutated gene and the nature of mutation for each mutant. Comparative analysis of phenotypes and genotypes of the mutants uncovered 202 new pathogenicity loci. Our findings demonstrate the effectiveness of our platform and provide new insights on the molecular basis of fungal pathogenesis. Our approach promises comprehensive functional genomics in filamentous fungi and beyond.

UR - http://www.scopus.com/inward/record.url?scp=34047150746&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34047150746&partnerID=8YFLogxK

U2 - 10.1038/ng2002

DO - 10.1038/ng2002

M3 - Article

VL - 39

SP - 561

EP - 565

JO - Nature Genetics

JF - Nature Genetics

SN - 1061-4036

IS - 4

ER -