Genome-wide functional analysis of pathogenicity genes in the rice blast fungus

Junhyun Jeon, Sook Young Park, Myoung Hwan Chi, Jaehyuk Choi, Jongsun Park, Hee-Sool Rho, Soonok Kim, Jaeduk Goh, Sungyong Yoo, Jinhee Choi, Ju Young Park, Mihwa Yi, Seonyoung Yang, Min Jung Kwon, Seong Sook Han, Byeong Ryun Kim, Chang Hyun Khang, Bongsoo Park, Se Eun Lim, Kyongyong JungSunghyung Kong, Maruthachalam Karunakaran, Hong Sik Oh, Hyojeong Kim, Seryun Kim, Jaejin Park, Soyoung Kang, Woo Bong Choi, Seogchan Kang, Yong Hwan Lee

Research output: Contribution to journalArticle

Abstract

Rapid translation of genome sequences into meaningful biological information hinges on the integration of multiple experimental and informatics methods into a cohesive platform. Despite the explosion in the number of genome sequences available, such a platform does not exist for filamentous fungi. Here we present the development and application of a functional genomics and informatics platform for a model plant pathogenic fungus, Magnaporthe oryzae. In total, we produced 21,070 mutants through large-scale insertional mutagenesis using Agrobacterium tumefaciens-mediated transformation. We used a high-throughput phenotype screening pipeline to detect disruption of seven phenotypes encompassing the fungal life cycle and identified the mutated gene and the nature of mutation for each mutant. Comparative analysis of phenotypes and genotypes of the mutants uncovered 202 new pathogenicity loci. Our findings demonstrate the effectiveness of our platform and provide new insights on the molecular basis of fungal pathogenesis. Our approach promises comprehensive functional genomics in filamentous fungi and beyond.

Original languageEnglish (US)
Pages (from-to)561-565
Number of pages5
JournalNature genetics
Volume39
Issue number4
DOIs
StatePublished - Apr 1 2007
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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  • Cite this

    Jeon, J., Park, S. Y., Chi, M. H., Choi, J., Park, J., Rho, H-S., Kim, S., Goh, J., Yoo, S., Choi, J., Park, J. Y., Yi, M., Yang, S., Kwon, M. J., Han, S. S., Kim, B. R., Khang, C. H., Park, B., Lim, S. E., ... Lee, Y. H. (2007). Genome-wide functional analysis of pathogenicity genes in the rice blast fungus. Nature genetics, 39(4), 561-565. https://doi.org/10.1038/ng2002