Genome-wide expression profile of LHON patients with the 11778 mutation

Khaled K. Abu-Amero, Mohammad Jaber, Ali Hellani, Thomas M. Bosley

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: To obtain a whole genome-expression profile in Leber hereditary optic neuropathy (LHON), patients with the 11 778 mitochondrial DNA mutation. Methods; RNA was extracted from leucocytes and cDNA reverse-transcribed and hybridised to Affymetrix Gene Chips. A principal-component analysis and the rate monotonic algorithm were performed, and a further analysis applied to genes with a twofold expression difference and p<0.015 between patients and controls. Results: The gene-expression profile of patients with the 11 778 mtDNA mutation was significantly different from controls. The most commonly upregulated genes (n=137) were found to be related to the cellular transport (13.8%; 19/137) and transcription (12.4%; 17/137). Similarly, the most commonly downregulated genes (n=152) were also related to the cellular transport (17.8%; 27/152) and transcription (18.4%; 28/152). None of the 13 mitochondrial coded genes and no structural mitochondrial nuclear-encoded genes were differentially expressed. Interestingly, OPA1 gene was downregulated in all LHON patients, which could lead to fragmentation of the mitochondrial network, dissipation of the mitochondrial membrane potential and disorganisation of the cristae. Conclusions: The presence of the 11 778 mtDNA mutation resulted in a unique gene-expression profile compared with controls. Downregulation of OPA1 may contribute to the pathogenesis of LHON.

Original languageEnglish (US)
Pages (from-to)256-259
Number of pages4
JournalBritish Journal of Ophthalmology
Volume94
Issue number2
DOIs
StatePublished - Feb 1 2010
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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