Genome-wide association study identifies genetic determinants of urine PCA3 levels in men

Zhuo Chen, Jielin Sun, Seong Tae Kim, Jack Groskopf, Junjie Feng, William B Isaacs, Roger S. Rittmaster, Lynn D. Condreay, Siqun Lilly Zheng, Jianfeng Xu

Research output: Contribution to journalArticle

Abstract

Prostate cancer gene 3 (PCA3) is a non-coding gene specifically overexpressed in prostate cancer (PCa) that has great potential as a clinical biomarker for predicting prostate biopsy outcome. However, genetic determinants of PCA3 expression level remain unknown. To investigate the association between genetic variants and PCA3 mRNA level, a genome-wide association study was conducted in 1371 men of European descent in the REduction by DUtasteride of prostate Cancer Events trial. First-voided urine specimens containing prostate cells were obtained after digital rectal examination. The PROGENSA PCA3 assay was used to determine PCA3 score in the urinary samples. A linear regression model was used to detect the associations between (single nucleotide polymorphisms) SNPs and PCA3 score under an additive genetic model, adjusting for age and population stratification. Two SNPs, rs10993994 in β-microseminoprotein at 10q11.23 and rs10424878 in kallikrein-related peptidase 2 at 19q13.33,were associated with PCA3 score at genome-wide significance level (P = 1.22 × 10-9 and 1.06 × 10-8, respectively). Men carrying the rs10993994 "T" allele or rs10424878 "A" allele had higher PCA3 score compared with men carrying rs10993994 "C" allele or rs10424878 "G" allele (β = 1.25 and 1.24, respectively). This is the first comprehensive search for genetic determinants of PCA3 score. The novel loci identified may provide insight into the molecular mechanisms of PCA3 expression as a potential marker of PCa.

Original languageEnglish (US)
Pages (from-to)448-453
Number of pages6
JournalNeoplasia
Volume15
Issue number4
DOIs
StatePublished - Apr 2013

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Neoplasm Genes
Genome-Wide Association Study
Prostatic Neoplasms
Urine
Alleles
Single Nucleotide Polymorphism
Prostate
Linear Models
Gene Expression
Digital Rectal Examination
Kallikreins
Genetic Models
Peptide Hydrolases
Biomarkers

ASJC Scopus subject areas

  • Cancer Research

Cite this

Genome-wide association study identifies genetic determinants of urine PCA3 levels in men. / Chen, Zhuo; Sun, Jielin; Kim, Seong Tae; Groskopf, Jack; Feng, Junjie; Isaacs, William B; Rittmaster, Roger S.; Condreay, Lynn D.; Zheng, Siqun Lilly; Xu, Jianfeng.

In: Neoplasia, Vol. 15, No. 4, 04.2013, p. 448-453.

Research output: Contribution to journalArticle

Chen, Z, Sun, J, Kim, ST, Groskopf, J, Feng, J, Isaacs, WB, Rittmaster, RS, Condreay, LD, Zheng, SL & Xu, J 2013, 'Genome-wide association study identifies genetic determinants of urine PCA3 levels in men', Neoplasia, vol. 15, no. 4, pp. 448-453. https://doi.org/10.1593/neo.122144
Chen, Zhuo ; Sun, Jielin ; Kim, Seong Tae ; Groskopf, Jack ; Feng, Junjie ; Isaacs, William B ; Rittmaster, Roger S. ; Condreay, Lynn D. ; Zheng, Siqun Lilly ; Xu, Jianfeng. / Genome-wide association study identifies genetic determinants of urine PCA3 levels in men. In: Neoplasia. 2013 ; Vol. 15, No. 4. pp. 448-453.
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