Genome-wide analysis reveals the vacuolar pH-stat of Saccharomyces cerevisiae

Christopher L. Brett, Laura Kallay, Zhaolin Hua, Richard Green, Anthony Chyou, Yongqiang Zhang, Todd R. Graham, Mark Donowitz, Rajini Rao

Research output: Contribution to journalArticle

Abstract

Protons, the smallest and most ubiquitous of ions, are central to physiological processes. Transmembrane proton gradients drive ATP synthesis, metabolite transport, receptor recycling and vesicle trafficking, while compartmental pH controls enzyme function. Despite this fundamental importance, the mechanisms underlying pH homeostasis are not entirely accounted for in any organelle or organism. We undertook a genome-wide survey of vacuole pH (pHv) in 4,606 single-gene deletion mutants of Saccharomyces cerevisiae under control, acid and alkali stress conditions to reveal the vacuolar pH-stat. Median pHv (5.27±0.13) was resistant to acid stress (5.28±0.14) but shifted significantly in response to alkali stress (5.83±0.13). Of 107 mutants that displayed aberrant pHv under more than one external pH condition, functional categories of transporters, membrane biogenesis and trafficking machinery were significantly enriched. Phospholipid flippases, encoded by the family of P4-type ATPases, emerged as pH regulators, as did the yeast ortholog of Niemann Pick Type C protein, implicated in sterol trafficking. An independent genetic screen revealed that correction of pHv dysregulation in a neo1ts mutant restored viability whereas cholesterol accumulation in human NPC1-/- fibroblasts diminished upon treatment with a proton ionophore. Furthermore, while it is established that lumenal pH affects trafficking, this study revealed a reciprocal link with many mutants defective in anterograde pathways being hyperacidic and retrograde pathway mutants with alkaline vacuoles. In these and other examples, pH perturbations emerge as a hitherto unrecognized phenotype that may contribute to the cellular basis of disease and offer potential therapeutic intervention through pH modulation.

Original languageEnglish (US)
Article numbere17619
JournalPLoS One
Volume6
Issue number3
DOIs
StatePublished - 2011

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Yeast
Saccharomyces cerevisiae
Genes
Alkalies
Genome
genome
Protons
Proton Ionophores
Acids
Membrane Transport Proteins
Sterols
Fibroblasts
mutants
Metabolites
Protein C
protons
Machinery
Adenosine Triphosphatases
Recycling
Phospholipids

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Brett, C. L., Kallay, L., Hua, Z., Green, R., Chyou, A., Zhang, Y., ... Rao, R. (2011). Genome-wide analysis reveals the vacuolar pH-stat of Saccharomyces cerevisiae. PLoS One, 6(3), [e17619]. https://doi.org/10.1371/journal.pone.0017619

Genome-wide analysis reveals the vacuolar pH-stat of Saccharomyces cerevisiae. / Brett, Christopher L.; Kallay, Laura; Hua, Zhaolin; Green, Richard; Chyou, Anthony; Zhang, Yongqiang; Graham, Todd R.; Donowitz, Mark; Rao, Rajini.

In: PLoS One, Vol. 6, No. 3, e17619, 2011.

Research output: Contribution to journalArticle

Brett, CL, Kallay, L, Hua, Z, Green, R, Chyou, A, Zhang, Y, Graham, TR, Donowitz, M & Rao, R 2011, 'Genome-wide analysis reveals the vacuolar pH-stat of Saccharomyces cerevisiae', PLoS One, vol. 6, no. 3, e17619. https://doi.org/10.1371/journal.pone.0017619
Brett, Christopher L. ; Kallay, Laura ; Hua, Zhaolin ; Green, Richard ; Chyou, Anthony ; Zhang, Yongqiang ; Graham, Todd R. ; Donowitz, Mark ; Rao, Rajini. / Genome-wide analysis reveals the vacuolar pH-stat of Saccharomyces cerevisiae. In: PLoS One. 2011 ; Vol. 6, No. 3.
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