Genome locations of temperature-sensitive mutants in glycoprotein gB of herpes simplex virus type 1

Neal DeLuca, Stanley Person, David J. Bzik, Wallace Snipes

Research output: Contribution to journalArticle

Abstract

A plasmid containing a herpes simplex virus type 1 (HSV-1) insert from strain KOS, prototypic coordinates 0.345 to 0.368 (3.45 kilobases) was mutagenized in vitro, and potential mutations were introduced into intact viral DNA by cotransfection. Functions normally associated with the glycoprotein gB are in the 1-9 complementation group, and the above coordinates include those that specify the gB glycoprotein gene. Following cotransfection, individual plaques were screened for temperature sensitivity (ts) of viral growth. A total of seven ts mutants was obtained, of which four were spurious mutations due to alterations outside the cloned sequences, presumably mediated by some aspect of the Ca-precipitation-cotranafection method. The remaining three did not complement known mutants of the 1-9 complementation group. These three mutants, along with tsJ12 (P. A. Schaffer, G. M. Aron, N. Biswal, and M. Benyesh-Melnick, 1973, Virology 52, 57-71) and tsJ33 (C.-T. Chu, D. S. Parris, R. A. F. Dixon, F. E. Farber, and P. A. Schaffer,1979, Virology 98, 168-181), were physically located by marker-rescue experiments to three different restriction fragments between 0.345 to 0.368 map units. Sodium dodecyl sulfate-gel electrophoresis was used to analyze the glycoproteins synthesized during continuous or pulse-chase labeling protocols. All five mutants were found to synthesize a precursor of gB but did not accumulate mature gB during a pulse, a chase, or continuous labeling at the nonpermissive temperature.

Original languageEnglish (US)
Pages (from-to)382-389
Number of pages8
JournalVirology
Volume137
Issue number2
DOIs
StatePublished - 1984
Externally publishedYes

Fingerprint

Human Herpesvirus 1
Glycoproteins
Virology
Genome
Temperature
Mutation
Viral DNA
Sodium Dodecyl Sulfate
Electrophoresis
Plasmids
Gels
Growth
Genes

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Genome locations of temperature-sensitive mutants in glycoprotein gB of herpes simplex virus type 1. / DeLuca, Neal; Person, Stanley; Bzik, David J.; Snipes, Wallace.

In: Virology, Vol. 137, No. 2, 1984, p. 382-389.

Research output: Contribution to journalArticle

DeLuca, Neal ; Person, Stanley ; Bzik, David J. ; Snipes, Wallace. / Genome locations of temperature-sensitive mutants in glycoprotein gB of herpes simplex virus type 1. In: Virology. 1984 ; Vol. 137, No. 2. pp. 382-389.
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