Genome expression analysis of Anopheles gambiae: Responses to injury, bacterial challenge, and malaria infection

George Dimopoulos, George K. Christophides, Stephan Meister, Jörg Schultz, Kevin P. White, Carolina Barillas-Mury, Fotis C. Kafatos

Research output: Contribution to journalArticle

Abstract

The complex gene expression responses of Anopheles gambiae to microbial and malaria challenges, injury, and oxidative stress (in the mosquito and/or a cultured cell line) were surveyed by using cDNA microarrays constructed from an EST-clone collection. The expression profiles were broadly subdivided into induced and down-regulated gene clusters. Gram+ and Gram- bacteria and microbial elicitors up-regulated a diverse set of genes, many belonging to the immunity class, and the response to malaria partially overlapped with this response. Oxidative stress activated a distinctive set of genes, mainly implicated in oxidoreductive processes. Injury up- and down-regulated gene clusters also were distinctive, prominently implicating glycolysis-related genes and citric acid cycle/oxidative phosphorylation/redox-mitochondrial functions, respectively. Cross-comparison of in vivo and in vitro responses indicated the existence of tightly coregulated gene groups that may correspond to gene pathways.

Original languageEnglish (US)
Pages (from-to)8814-8819
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number13
DOIs
StatePublished - Jun 25 2002

ASJC Scopus subject areas

  • General

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