@inbook{27ea5be56fa243268dc4d65ecb350083,
title = "Genome editing in zebrafish using CRISPR-Cas9: Applications for developmental toxicology",
abstract = "Environment-gene interactions have a powerful impact on embryo development. The ability to precisely edit the genome makes it possible to address questions concerning the specific roles that genes or variants play in modulating the response to environmental challenges. In this chapter, we provide a simplified protocol using CRISPR-Cas9 ribonucleoproteins for genome editing in the zebrafish model organism. The genetic manipulation can then be coupled with chemical screens to identify and understand the mechanism behind toxicants or compounds that modulate development.",
keywords = "CRISPR, Cas9, Clustered regularly interspaced short palindromic repeats, Genomic editing, Morpholino, Zebrafish",
author = "Warner, {Brendon K.} and Alder, {Jonathan K.} and Arminda Suli",
note = "Funding Information: We would like to thank David J. Grunwald and Michael J. Jurynec at the University of Utah for providing the initial Cas9 purification protocol, which we have modified to the existing protocol. This work was supported by NIH grants (HL113105) to Jonathan K. Alder. Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",
year = "2019",
doi = "10.1007/978-1-4939-9182-2_16",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "235--250",
booktitle = "Methods in Molecular Biology",
}