Genome editing in zebrafish using CRISPR-Cas9: Applications for developmental toxicology

Brendon K. Warner, Jonathan Alder, Arminda Suli

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Environment-gene interactions have a powerful impact on embryo development. The ability to precisely edit the genome makes it possible to address questions concerning the specific roles that genes or variants play in modulating the response to environmental challenges. In this chapter, we provide a simplified protocol using CRISPR-Cas9 ribonucleoproteins for genome editing in the zebrafish model organism. The genetic manipulation can then be coupled with chemical screens to identify and understand the mechanism behind toxicants or compounds that modulate development.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages235-250
Number of pages16
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1965
ISSN (Print)1064-3745

Keywords

  • Cas9
  • Clustered regularly interspaced short palindromic repeats
  • CRISPR
  • Genomic editing
  • Morpholino
  • Zebrafish

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Warner, B. K., Alder, J., & Suli, A. (2019). Genome editing in zebrafish using CRISPR-Cas9: Applications for developmental toxicology. In Methods in Molecular Biology (pp. 235-250). (Methods in Molecular Biology; Vol. 1965). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9182-2_16