Genome editing in zebrafish using CRISPR-Cas9: Applications for developmental toxicology

Brendon K. Warner, Jonathan K. Alder, Arminda Suli

Research output: Chapter in Book/Report/Conference proceedingChapter


Environment-gene interactions have a powerful impact on embryo development. The ability to precisely edit the genome makes it possible to address questions concerning the specific roles that genes or variants play in modulating the response to environmental challenges. In this chapter, we provide a simplified protocol using CRISPR-Cas9 ribonucleoproteins for genome editing in the zebrafish model organism. The genetic manipulation can then be coupled with chemical screens to identify and understand the mechanism behind toxicants or compounds that modulate development.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages16
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Cas9
  • Clustered regularly interspaced short palindromic repeats
  • Genomic editing
  • Morpholino
  • Zebrafish

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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