Abstract
Environment-gene interactions have a powerful impact on embryo development. The ability to precisely edit the genome makes it possible to address questions concerning the specific roles that genes or variants play in modulating the response to environmental challenges. In this chapter, we provide a simplified protocol using CRISPR-Cas9 ribonucleoproteins for genome editing in the zebrafish model organism. The genetic manipulation can then be coupled with chemical screens to identify and understand the mechanism behind toxicants or compounds that modulate development.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 235-250 |
| Number of pages | 16 |
| DOIs | |
| State | Published - Jan 1 2019 |
| Externally published | Yes |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1965 |
| ISSN (Print) | 1064-3745 |
Keywords
- Cas9
- Clustered regularly interspaced short palindromic repeats
- CRISPR
- Genomic editing
- Morpholino
- Zebrafish
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Fingerprint Dive into the research topics of 'Genome editing in zebrafish using CRISPR-Cas9: Applications for developmental toxicology'. Together they form a unique fingerprint.
Cite this
Warner, B. K., Alder, J., & Suli, A. (2019). Genome editing in zebrafish using CRISPR-Cas9: Applications for developmental toxicology. In Methods in Molecular Biology (pp. 235-250). (Methods in Molecular Biology; Vol. 1965). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9182-2_16