Genital herpes evaluation by quantitative TaqMan PCR: Correlating single detection and quantity of HSV-2 DNA in cervicovaginal lavage fluids with cross-sectional and longitudinal clinical data

Bulbulgul Aumakhan, Andrew Hardick, Thomas C Quinn, Oliver B. Laeyendecker, Stephen J Gange, Christopher Beyrer, Christopher Cox, Kathryn Anastos, Mardge Cohen, Ruth M. Greenblatt, Daniel J. Merenstein, Howard Minkoff, Marek Nowicki, Charlotte A Gaydos

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Abstract

Objective. To evaluate the utility of a single quantitative PCR (qPCR) measurement of HSV (HSV-1&2) DNA in cervicovaginal lavage (CVL) specimens collected from women with predominantly chronic HSV-2 infection in assessing genital HSV shedding and the clinical course of genital herpes (GH) within a cohort with semiannual schedule of follow up and collection of specimens. Methods. Two previously described methods used for detection of HSV DNA in mucocutaneous swab samples were adapted for quantification of HSV DNA in CVLs. Single CVL specimens from 509 women were tested. Presence and quantity of CVL HSV DNA were explored in relation to observed cross-sectional and longitudinal clinical data. Results. The PCR assay was sensitive and reproducible with a limit of quantification of ∼50 copies per milliliter of CVL. Overall, 7% of the samples were positive for HSV-2 DNA with median log10HSV-2 DNA copy number of 3.9 (IQR: 2.6-5.7). No HSV-1 was detected. Presence and quantity of HSV-2 DNA in CVL directly correlated with the clinical signs and symptoms of presence of active symptomatic disease with frequent recurrences. Conclusion. Single qPCR measurement of HSV DNA in CVL fluids of women with chronic HSV-2 infection provided useful information for assessing GH in the setting of infrequent sampling of specimens. Observed positive correlation of the presence and quantity of HSV-2 DNA with the presence of active and more severe course of HSV-2 infection may have clinical significance in the evaluation and management of HSV-2 infected patients.

Original languageEnglish (US)
Article number328
JournalVirology Journal
Volume7
DOIs
StatePublished - 2010

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Herpes Genitalis
Human Herpesvirus 2
Therapeutic Irrigation
Polymerase Chain Reaction
DNA
Human Herpesvirus 1
Infection
Specimen Handling
Signs and Symptoms
Appointments and Schedules
Recurrence

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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Genital herpes evaluation by quantitative TaqMan PCR : Correlating single detection and quantity of HSV-2 DNA in cervicovaginal lavage fluids with cross-sectional and longitudinal clinical data. / Aumakhan, Bulbulgul; Hardick, Andrew; Quinn, Thomas C; Laeyendecker, Oliver B.; Gange, Stephen J; Beyrer, Christopher; Cox, Christopher; Anastos, Kathryn; Cohen, Mardge; Greenblatt, Ruth M.; Merenstein, Daniel J.; Minkoff, Howard; Nowicki, Marek; Gaydos, Charlotte A.

In: Virology Journal, Vol. 7, 328, 2010.

Research output: Contribution to journalArticle

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title = "Genital herpes evaluation by quantitative TaqMan PCR: Correlating single detection and quantity of HSV-2 DNA in cervicovaginal lavage fluids with cross-sectional and longitudinal clinical data",
abstract = "Objective. To evaluate the utility of a single quantitative PCR (qPCR) measurement of HSV (HSV-1&2) DNA in cervicovaginal lavage (CVL) specimens collected from women with predominantly chronic HSV-2 infection in assessing genital HSV shedding and the clinical course of genital herpes (GH) within a cohort with semiannual schedule of follow up and collection of specimens. Methods. Two previously described methods used for detection of HSV DNA in mucocutaneous swab samples were adapted for quantification of HSV DNA in CVLs. Single CVL specimens from 509 women were tested. Presence and quantity of CVL HSV DNA were explored in relation to observed cross-sectional and longitudinal clinical data. Results. The PCR assay was sensitive and reproducible with a limit of quantification of ∼50 copies per milliliter of CVL. Overall, 7{\%} of the samples were positive for HSV-2 DNA with median log10HSV-2 DNA copy number of 3.9 (IQR: 2.6-5.7). No HSV-1 was detected. Presence and quantity of HSV-2 DNA in CVL directly correlated with the clinical signs and symptoms of presence of active symptomatic disease with frequent recurrences. Conclusion. Single qPCR measurement of HSV DNA in CVL fluids of women with chronic HSV-2 infection provided useful information for assessing GH in the setting of infrequent sampling of specimens. Observed positive correlation of the presence and quantity of HSV-2 DNA with the presence of active and more severe course of HSV-2 infection may have clinical significance in the evaluation and management of HSV-2 infected patients.",
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doi = "10.1186/1743-422X-7-328",
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T2 - Correlating single detection and quantity of HSV-2 DNA in cervicovaginal lavage fluids with cross-sectional and longitudinal clinical data

AU - Aumakhan, Bulbulgul

AU - Hardick, Andrew

AU - Quinn, Thomas C

AU - Laeyendecker, Oliver B.

AU - Gange, Stephen J

AU - Beyrer, Christopher

AU - Cox, Christopher

AU - Anastos, Kathryn

AU - Cohen, Mardge

AU - Greenblatt, Ruth M.

AU - Merenstein, Daniel J.

AU - Minkoff, Howard

AU - Nowicki, Marek

AU - Gaydos, Charlotte A

PY - 2010

Y1 - 2010

N2 - Objective. To evaluate the utility of a single quantitative PCR (qPCR) measurement of HSV (HSV-1&2) DNA in cervicovaginal lavage (CVL) specimens collected from women with predominantly chronic HSV-2 infection in assessing genital HSV shedding and the clinical course of genital herpes (GH) within a cohort with semiannual schedule of follow up and collection of specimens. Methods. Two previously described methods used for detection of HSV DNA in mucocutaneous swab samples were adapted for quantification of HSV DNA in CVLs. Single CVL specimens from 509 women were tested. Presence and quantity of CVL HSV DNA were explored in relation to observed cross-sectional and longitudinal clinical data. Results. The PCR assay was sensitive and reproducible with a limit of quantification of ∼50 copies per milliliter of CVL. Overall, 7% of the samples were positive for HSV-2 DNA with median log10HSV-2 DNA copy number of 3.9 (IQR: 2.6-5.7). No HSV-1 was detected. Presence and quantity of HSV-2 DNA in CVL directly correlated with the clinical signs and symptoms of presence of active symptomatic disease with frequent recurrences. Conclusion. Single qPCR measurement of HSV DNA in CVL fluids of women with chronic HSV-2 infection provided useful information for assessing GH in the setting of infrequent sampling of specimens. Observed positive correlation of the presence and quantity of HSV-2 DNA with the presence of active and more severe course of HSV-2 infection may have clinical significance in the evaluation and management of HSV-2 infected patients.

AB - Objective. To evaluate the utility of a single quantitative PCR (qPCR) measurement of HSV (HSV-1&2) DNA in cervicovaginal lavage (CVL) specimens collected from women with predominantly chronic HSV-2 infection in assessing genital HSV shedding and the clinical course of genital herpes (GH) within a cohort with semiannual schedule of follow up and collection of specimens. Methods. Two previously described methods used for detection of HSV DNA in mucocutaneous swab samples were adapted for quantification of HSV DNA in CVLs. Single CVL specimens from 509 women were tested. Presence and quantity of CVL HSV DNA were explored in relation to observed cross-sectional and longitudinal clinical data. Results. The PCR assay was sensitive and reproducible with a limit of quantification of ∼50 copies per milliliter of CVL. Overall, 7% of the samples were positive for HSV-2 DNA with median log10HSV-2 DNA copy number of 3.9 (IQR: 2.6-5.7). No HSV-1 was detected. Presence and quantity of HSV-2 DNA in CVL directly correlated with the clinical signs and symptoms of presence of active symptomatic disease with frequent recurrences. Conclusion. Single qPCR measurement of HSV DNA in CVL fluids of women with chronic HSV-2 infection provided useful information for assessing GH in the setting of infrequent sampling of specimens. Observed positive correlation of the presence and quantity of HSV-2 DNA with the presence of active and more severe course of HSV-2 infection may have clinical significance in the evaluation and management of HSV-2 infected patients.

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