Genital anaerobic bacterial overgrowth and the prepex male circumcision device, rakai, Uganda

Cindy M. Liu, Jessica Prodger, Aaron A Tobian, David Serwadda, Ronald M. Galiwango, Fred Nalugoda, Nehemiah Kighoma, Joshua Mwinike, Margaret Anyokorit, Lance B. Price, Maria J Wawer, Godfrey Kigozi, Ronald H Gray

Research output: Contribution to journalArticle

Abstract

The PrePex circumcision device causes ischemic necrosis of the foreskin, raising concerns of anaerobic overgrowth. We compared the subpreputial microbiome of 2 men 7 days after PrePex device placement to that of 145 uncircumcised men in Rakai, Uganda, using 16S ribosomal (rRNA) RNA gene-based quantitative polymerase chain reaction analysis and sequencing. Pre-Pex users had higher absolute abundance of all bacteria than uncircumcised men (P = .001), largely due to increased numbers of the following anaerobes: Porphyromonas (5.2 × 107 16S rRNA gene copies/swab in the PrePex group and 1.1 × 106 16S rRNA gene copies/swab in uncircumcised men; P = .002), Peptoniphilus (1.0 × 107 and 1.8 × 106 16S rRNA gene copies/swab, respectively; P < .05), Anaerococcus (1.0 × 107 and 1.1 × 106 16S rRNA gene copies/swab, respectively; P < .001), and Campylobacter ureolyticus (1.7 × 105 and 1.6 × 10716S rRNA gene copies/swab, respectively; P < .001). The PrePex-associated increase in anaerobes may account for unpleasant odor and a possible heightened risk of tetanus.

Original languageEnglish (US)
Pages (from-to)595-598
Number of pages4
JournalJournal of Infectious Diseases
Volume214
Issue number4
DOIs
StatePublished - Aug 15 2016

Fingerprint

Male Circumcision
Uganda
rRNA Genes
Equipment and Supplies
Porphyromonas
16S Ribosomal RNA
Foreskin
Campylobacter
Microbiota
Tetanus
Necrosis
Bacteria
Polymerase Chain Reaction

Keywords

  • Anaerobes
  • Male circumcision
  • Penile microbiota
  • PrePex device
  • Tetanus
  • Uganda

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Genital anaerobic bacterial overgrowth and the prepex male circumcision device, rakai, Uganda. / Liu, Cindy M.; Prodger, Jessica; Tobian, Aaron A; Serwadda, David; Galiwango, Ronald M.; Nalugoda, Fred; Kighoma, Nehemiah; Mwinike, Joshua; Anyokorit, Margaret; Price, Lance B.; Wawer, Maria J; Kigozi, Godfrey; Gray, Ronald H.

In: Journal of Infectious Diseases, Vol. 214, No. 4, 15.08.2016, p. 595-598.

Research output: Contribution to journalArticle

Liu, CM, Prodger, J, Tobian, AA, Serwadda, D, Galiwango, RM, Nalugoda, F, Kighoma, N, Mwinike, J, Anyokorit, M, Price, LB, Wawer, MJ, Kigozi, G & Gray, RH 2016, 'Genital anaerobic bacterial overgrowth and the prepex male circumcision device, rakai, Uganda', Journal of Infectious Diseases, vol. 214, no. 4, pp. 595-598. https://doi.org/10.1093/infdis/jiw182
Liu, Cindy M. ; Prodger, Jessica ; Tobian, Aaron A ; Serwadda, David ; Galiwango, Ronald M. ; Nalugoda, Fred ; Kighoma, Nehemiah ; Mwinike, Joshua ; Anyokorit, Margaret ; Price, Lance B. ; Wawer, Maria J ; Kigozi, Godfrey ; Gray, Ronald H. / Genital anaerobic bacterial overgrowth and the prepex male circumcision device, rakai, Uganda. In: Journal of Infectious Diseases. 2016 ; Vol. 214, No. 4. pp. 595-598.
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