Genital anaerobic bacterial overgrowth and the prepex male circumcision device, rakai, Uganda

Cindy M. Liu; Jessica L. Prodger; Aaron A.R. Tobian; David Serwadda; Ronald M. Galiwango; Fred Nalugoda; Nehemiah Kighoma; Joshua Mwinike; Margaret Anyokorit; Lance B. Price; Maria J. Wawer; Godfrey Kigozi; Ronald H. Gray

doi:10.1093/infdis/jiw182

Genital anaerobic bacterial overgrowth and the prepex male circumcision device, rakai, Uganda

Cindy M. Liu, Jessica L. Prodger, Aaron A.R. Tobian, David Serwadda, Ronald M. Galiwango, Fred Nalugoda, Nehemiah Kighoma, Joshua Mwinike, Margaret Anyokorit, Lance B. Price, Maria J. Wawer, Godfrey Kigozi, Ronald H. Gray

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The PrePex circumcision device causes ischemic necrosis of the foreskin, raising concerns of anaerobic overgrowth. We compared the subpreputial microbiome of 2 men 7 days after PrePex device placement to that of 145 uncircumcised men in Rakai, Uganda, using 16S ribosomal (rRNA) RNA gene-based quantitative polymerase chain reaction analysis and sequencing. Pre-Pex users had higher absolute abundance of all bacteria than uncircumcised men (P = .001), largely due to increased numbers of the following anaerobes: Porphyromonas (5.2 × 107 16S rRNA gene copies/swab in the PrePex group and 1.1 × 106 16S rRNA gene copies/swab in uncircumcised men; P = .002), Peptoniphilus (1.0 × 107 and 1.8 × 106 16S rRNA gene copies/swab, respectively; P < .05), Anaerococcus (1.0 × 107 and 1.1 × 106 16S rRNA gene copies/swab, respectively; P < .001), and Campylobacter ureolyticus (1.7 × 105 and 1.6 × 10716S rRNA gene copies/swab, respectively; P < .001). The PrePex-associated increase in anaerobes may account for unpleasant odor and a possible heightened risk of tetanus.

Original language	English (US)
Pages (from-to)	595-598
Number of pages	4
Journal	Journal of Infectious Diseases
Volume	214
Issue number	4
DOIs	https://doi.org/10.1093/infdis/jiw182
State	Published - Aug 15 2016

Keywords

Anaerobes
Male circumcision
Penile microbiota
PrePex device
Tetanus
Uganda

ASJC Scopus subject areas

General Medicine

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10.1093/infdis/jiw182

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Liu, C. M., Prodger, J. L., Tobian, A. A. R., Serwadda, D., Galiwango, R. M., Nalugoda, F., Kighoma, N., Mwinike, J., Anyokorit, M., Price, L. B., Wawer, M. J., Kigozi, G., & Gray, R. H. (2016). Genital anaerobic bacterial overgrowth and the prepex male circumcision device, rakai, Uganda. Journal of Infectious Diseases, 214(4), 595-598. https://doi.org/10.1093/infdis/jiw182

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abstract = "The PrePex circumcision device causes ischemic necrosis of the foreskin, raising concerns of anaerobic overgrowth. We compared the subpreputial microbiome of 2 men 7 days after PrePex device placement to that of 145 uncircumcised men in Rakai, Uganda, using 16S ribosomal (rRNA) RNA gene-based quantitative polymerase chain reaction analysis and sequencing. Pre-Pex users had higher absolute abundance of all bacteria than uncircumcised men (P = .001), largely due to increased numbers of the following anaerobes: Porphyromonas (5.2 × 107 16S rRNA gene copies/swab in the PrePex group and 1.1 × 106 16S rRNA gene copies/swab in uncircumcised men; P = .002), Peptoniphilus (1.0 × 107 and 1.8 × 106 16S rRNA gene copies/swab, respectively; P < .05), Anaerococcus (1.0 × 107 and 1.1 × 106 16S rRNA gene copies/swab, respectively; P < .001), and Campylobacter ureolyticus (1.7 × 105 and 1.6 × 10716S rRNA gene copies/swab, respectively; P < .001). The PrePex-associated increase in anaerobes may account for unpleasant odor and a possible heightened risk of tetanus.",

keywords = "Anaerobes, Male circumcision, Penile microbiota, PrePex device, Tetanus, Uganda",

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AU - Tobian, Aaron A.R.

AU - Serwadda, David

AU - Galiwango, Ronald M.

AU - Nalugoda, Fred

AU - Kighoma, Nehemiah

AU - Mwinike, Joshua

AU - Anyokorit, Margaret

AU - Price, Lance B.

AU - Wawer, Maria J.

AU - Kigozi, Godfrey

AU - Gray, Ronald H.

N1 - Publisher Copyright: © The Author 2016.

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N2 - The PrePex circumcision device causes ischemic necrosis of the foreskin, raising concerns of anaerobic overgrowth. We compared the subpreputial microbiome of 2 men 7 days after PrePex device placement to that of 145 uncircumcised men in Rakai, Uganda, using 16S ribosomal (rRNA) RNA gene-based quantitative polymerase chain reaction analysis and sequencing. Pre-Pex users had higher absolute abundance of all bacteria than uncircumcised men (P = .001), largely due to increased numbers of the following anaerobes: Porphyromonas (5.2 × 107 16S rRNA gene copies/swab in the PrePex group and 1.1 × 106 16S rRNA gene copies/swab in uncircumcised men; P = .002), Peptoniphilus (1.0 × 107 and 1.8 × 106 16S rRNA gene copies/swab, respectively; P < .05), Anaerococcus (1.0 × 107 and 1.1 × 106 16S rRNA gene copies/swab, respectively; P < .001), and Campylobacter ureolyticus (1.7 × 105 and 1.6 × 10716S rRNA gene copies/swab, respectively; P < .001). The PrePex-associated increase in anaerobes may account for unpleasant odor and a possible heightened risk of tetanus.

AB - The PrePex circumcision device causes ischemic necrosis of the foreskin, raising concerns of anaerobic overgrowth. We compared the subpreputial microbiome of 2 men 7 days after PrePex device placement to that of 145 uncircumcised men in Rakai, Uganda, using 16S ribosomal (rRNA) RNA gene-based quantitative polymerase chain reaction analysis and sequencing. Pre-Pex users had higher absolute abundance of all bacteria than uncircumcised men (P = .001), largely due to increased numbers of the following anaerobes: Porphyromonas (5.2 × 107 16S rRNA gene copies/swab in the PrePex group and 1.1 × 106 16S rRNA gene copies/swab in uncircumcised men; P = .002), Peptoniphilus (1.0 × 107 and 1.8 × 106 16S rRNA gene copies/swab, respectively; P < .05), Anaerococcus (1.0 × 107 and 1.1 × 106 16S rRNA gene copies/swab, respectively; P < .001), and Campylobacter ureolyticus (1.7 × 105 and 1.6 × 10716S rRNA gene copies/swab, respectively; P < .001). The PrePex-associated increase in anaerobes may account for unpleasant odor and a possible heightened risk of tetanus.

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Genital anaerobic bacterial overgrowth and the prepex male circumcision device, rakai, Uganda

Abstract

Keywords

ASJC Scopus subject areas

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