Genital anaerobic bacterial overgrowth and the prepex male circumcision device, rakai, Uganda

Cindy M. Liu, Jessica L. Prodger, Aaron A.R. Tobian, David Serwadda, Ronald M. Galiwango, Fred Nalugoda, Nehemiah Kighoma, Joshua Mwinike, Margaret Anyokorit, Lance B. Price, Maria J. Wawer, Godfrey Kigozi, Ronald H. Gray

Research output: Contribution to journalArticle

Abstract

The PrePex circumcision device causes ischemic necrosis of the foreskin, raising concerns of anaerobic overgrowth. We compared the subpreputial microbiome of 2 men 7 days after PrePex device placement to that of 145 uncircumcised men in Rakai, Uganda, using 16S ribosomal (rRNA) RNA gene-based quantitative polymerase chain reaction analysis and sequencing. Pre-Pex users had higher absolute abundance of all bacteria than uncircumcised men (P = .001), largely due to increased numbers of the following anaerobes: Porphyromonas (5.2 × 107 16S rRNA gene copies/swab in the PrePex group and 1.1 × 106 16S rRNA gene copies/swab in uncircumcised men; P = .002), Peptoniphilus (1.0 × 107 and 1.8 × 106 16S rRNA gene copies/swab, respectively; P < .05), Anaerococcus (1.0 × 107 and 1.1 × 106 16S rRNA gene copies/swab, respectively; P < .001), and Campylobacter ureolyticus (1.7 × 105 and 1.6 × 10716S rRNA gene copies/swab, respectively; P < .001). The PrePex-associated increase in anaerobes may account for unpleasant odor and a possible heightened risk of tetanus.

Original languageEnglish (US)
Pages (from-to)595-598
Number of pages4
JournalJournal of Infectious Diseases
Volume214
Issue number4
DOIs
StatePublished - Aug 15 2016

Keywords

  • Anaerobes
  • Male circumcision
  • Penile microbiota
  • PrePex device
  • Tetanus
  • Uganda

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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  • Cite this

    Liu, C. M., Prodger, J. L., Tobian, A. A. R., Serwadda, D., Galiwango, R. M., Nalugoda, F., Kighoma, N., Mwinike, J., Anyokorit, M., Price, L. B., Wawer, M. J., Kigozi, G., & Gray, R. H. (2016). Genital anaerobic bacterial overgrowth and the prepex male circumcision device, rakai, Uganda. Journal of Infectious Diseases, 214(4), 595-598. https://doi.org/10.1093/infdis/jiw182