Genetics and biology of human ovarian terratomas. II. Molecular analysis of origin of nondisjunction and gene-centromere mapping of chromosome I markers

R. Deka, A. Chakravarti, U. Surti, E. Hauselman, J. Reefer, P. P. Majumder, R. E. Ferrell

Research output: Contribution to journalArticlepeer-review

Abstract

Chromosomal heteromorphisms and DNA polymorphisms have been utilized to identify the mechanisms that lead to formation of human ovarian teratomas and to construct a gene-centromere map of chromosome 1 by using those teratomas that arise by meiotic nondisjunction. OF 61 genetically informative ovarian teratomas, 21.3% arose by nondisjunction at meiosis I, and 39.3% arose by meiosis II nondisjunction. Eight polymorphic marker loci on chromosome 1p and one marker on 1q were used to estimate a gene-centromere map. The results show clear linkage of the most proximal 1p marker (NRAS) and the most proximal 1q marker (D1S61) to the centromere at a distance of 14 cM and 20 cM, respectively. Estimated gene-centromere distances suggest that, while recombination occurs normally in ovarian teratomas arising by meiosis II errors, ovarian teratomas arising by meiosis I nondisjunction have altered patterns of recombination. Furthermore, the estimated map demonstrates clear evidence of chiasma interference. Our results suggest that ovarian teratomas can provide a rapid method for mapping genes relative to the centromere.

Original languageEnglish (US)
Pages (from-to)644-655
Number of pages12
JournalAmerican journal of human genetics
Volume47
Issue number4
StatePublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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