Genetically encoded molecular probes to visualize and perturb signaling dynamics in living biological systems

Vedangi Sample, Sohum Mehta, Jin Zhang

Research output: Contribution to journalArticlepeer-review

Abstract

In this Commentary, we discuss two sets of genetically encoded molecular tools that have significantly enhanced our ability to observe and manipulate complex biochemical processes in their native context and that have been essential in deepening our molecular understanding of how intracellular signaling networks function. In particular, genetically encoded biosensors are widely used to directly visualize signaling events in living cells, and we highlight several examples of basic biosensor designs that have enabled researchers to capture the spatial and temporal dynamics of numerous signaling molecules, including second messengers and signaling enzymes, with remarkable detail. Similarly, we discuss a number of genetically encoded biochemical perturbation techniques that are being used to manipulate the activity of various signaling molecules with far greater spatial and temporal selectivity than can be achieved using standard pharmacological or genetic techniques, focusing specifically on examples of chemically driven and light-inducible perturbation strategies. We then describe recent efforts to combine these diverse and powerful molecular tools into a unified platform that can be used to elucidate the molecular details of biological processes that may potentially extend well beyond the realm of signal transduction.

Original languageEnglish (US)
Pages (from-to)1151-1160
Number of pages10
JournalJournal of Cell Science
Volume127
Issue number6
DOIs
StatePublished - Mar 2014

Keywords

  • Biosensors
  • FRET
  • Live cell imaging
  • Signaling
  • Targeted perturbations

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

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