Genetically diverse low pathogenicity avian influenza A virus subtypes co-circulate among poultry in Bangladesh

Nancy A. Gerloff, Salah Uddin Khan, Natosha Zanders, Amanda Balish, Najmul Haider, Ausraful Islam, Sukanta Chowdhury, Mahmudur Ziaur Rahman, Ainul Haque, Parviez Hosseini, Emily Gurley, Stephen P. Luby, David E. Wentworth, Ruben O. Donis, Katharine Sturm-Ramirez, C. Todd Davis

Research output: Contribution to journalArticle

Abstract

Influenza virus surveillance, poultry outbreak investigations and genomic sequencing were assessed to understand the ecology and evolution of low pathogenicity avian influenza (LPAI) A viruses in Bangladesh from 2007 to 2013. We analyzed 506 avian specimens collected from poultry in live bird markets and backyard flocks to identify influenza A viruses. Virus isolation-positive specimens (n = 50) were subtyped and their coding-complete genomes were sequenced. The most frequently identified subtypes among LPAI isolates were H9N2, H11N3, H4N6, and H1N1. Less frequently detected subtypes included H1N3, H2N4, H3N2, H3N6, H3N8, H4N2, H5N2, H6N1, H6N7, and H7N9. Gene sequences were compared to publicly available sequences using phylogenetic inference approaches. Among the 14 subtypes identified, the majority of viral gene segments were most closely related to poultry or wild bird viruses commonly found in Southeast Asia, Europe, and/or northern Africa. LPAI subtypes were distributed over several geographic locations in Bangladesh, and surface and internal protein gene segments clustered phylogenetically with a diverse number of viral subtypes suggesting extensive reassortment among these LPAI viruses. H9N2 subtype viruses differed from other LPAI subtypes because genes from these viruses consistently clustered together, indicating this subtype is enzootic in Bangladesh. The H9N2 strains identified in Bangladesh were phylogenetically and antigenically related to previous human-derived H9N2 viruses detected in Bangladesh representing a potential source for human infection. In contrast, the circulating LPAI H5N2 and H7N9 viruses were both phylogenetically and antigenically unrelated to H5 viruses identified previously in humans in Bangladesh and H7N9 strains isolated from humans in China. In Bangladesh, domestic poultry sold in live bird markets carried a wide range of LPAI virus subtypes and a high diversity of genotypes. These findings, combined with the seven year timeframe of sampling, indicate a continuous circulation of these viruses in the country.

Original languageEnglish (US)
Article numbere0152131
JournalPLoS One
Volume11
Issue number3
DOIs
StatePublished - Mar 1 2016
Externally publishedYes

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Poultry
Influenza in Birds
Bangladesh
Influenza A virus
Viruses
Virulence
poultry
pathogenicity
viruses
avian influenza
Orthomyxoviridae
H9N2 Subtype Influenza A Virus
Birds
H5N2 Subtype Influenza A Virus
H7N9 Subtype Influenza A Virus
Genes
markets
outbreak investigation
Northern Africa
Geographic Locations

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Gerloff, N. A., Khan, S. U., Zanders, N., Balish, A., Haider, N., Islam, A., ... Davis, C. T. (2016). Genetically diverse low pathogenicity avian influenza A virus subtypes co-circulate among poultry in Bangladesh. PLoS One, 11(3), [e0152131]. https://doi.org/10.1371/journal.pone.0152131

Genetically diverse low pathogenicity avian influenza A virus subtypes co-circulate among poultry in Bangladesh. / Gerloff, Nancy A.; Khan, Salah Uddin; Zanders, Natosha; Balish, Amanda; Haider, Najmul; Islam, Ausraful; Chowdhury, Sukanta; Rahman, Mahmudur Ziaur; Haque, Ainul; Hosseini, Parviez; Gurley, Emily; Luby, Stephen P.; Wentworth, David E.; Donis, Ruben O.; Sturm-Ramirez, Katharine; Davis, C. Todd.

In: PLoS One, Vol. 11, No. 3, e0152131, 01.03.2016.

Research output: Contribution to journalArticle

Gerloff, NA, Khan, SU, Zanders, N, Balish, A, Haider, N, Islam, A, Chowdhury, S, Rahman, MZ, Haque, A, Hosseini, P, Gurley, E, Luby, SP, Wentworth, DE, Donis, RO, Sturm-Ramirez, K & Davis, CT 2016, 'Genetically diverse low pathogenicity avian influenza A virus subtypes co-circulate among poultry in Bangladesh', PLoS One, vol. 11, no. 3, e0152131. https://doi.org/10.1371/journal.pone.0152131
Gerloff, Nancy A. ; Khan, Salah Uddin ; Zanders, Natosha ; Balish, Amanda ; Haider, Najmul ; Islam, Ausraful ; Chowdhury, Sukanta ; Rahman, Mahmudur Ziaur ; Haque, Ainul ; Hosseini, Parviez ; Gurley, Emily ; Luby, Stephen P. ; Wentworth, David E. ; Donis, Ruben O. ; Sturm-Ramirez, Katharine ; Davis, C. Todd. / Genetically diverse low pathogenicity avian influenza A virus subtypes co-circulate among poultry in Bangladesh. In: PLoS One. 2016 ; Vol. 11, No. 3.
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