Genetic variation of vibrio cholerae during outbreaks, Bangladesh, 2010-2011

Shah M. Rashed, Andrew S. Azman, Munirul Alam, Shan Li, David A. Sack, J. Glenn Morris, Ira Longini, Abul Kasem Siddique, Anwarul Iqbal, Anwar Huq, Rita R. Colwell, R. Bradley Sack, O. Colin Stine

Research output: Contribution to journalArticle

Abstract

Cholera remains a major public health problem. To compare the relative contribution of strains from the environment with strains isolated from patients during outbreaks, we performed multilocus variable tandem repeat analyses on samples collected during the 2010 and 2011 outbreak seasons in 2 geographically distinct areas of Bangladesh. A total of 222 environmental and clinical isolates of V. cholerae O1 were systematically collected from Chhatak and Mathbaria. In Chhatak, 75 of 79 isolates were from the same clonal complex, in which extensive differentiation was found in a temporally consistent pattern of successive mutations at single loci. A total of 59 isolates were collected from 6 persons; most isolates from 1 person differed by sequential single-locus mutations. In Mathbaria, 60 of 84 isolates represented 2 separate clonal complexes. The small number of genetic lineages in isolates from patients, compared with those from the environment, is consistent with accelerated transmission of some strains among humans during an outbreak.

Original languageEnglish (US)
Pages (from-to)54-60
Number of pages7
JournalEmerging Infectious Diseases
Volume20
Issue number1
DOIs
StatePublished - 2014
Externally publishedYes

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Vibrio cholerae
Bangladesh
Disease Outbreaks
Vibrio cholerae O1
Minisatellite Repeats
Mutation
Cholera
Public Health

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Epidemiology

Cite this

Genetic variation of vibrio cholerae during outbreaks, Bangladesh, 2010-2011. / Rashed, Shah M.; Azman, Andrew S.; Alam, Munirul; Li, Shan; Sack, David A.; Morris, J. Glenn; Longini, Ira; Siddique, Abul Kasem; Iqbal, Anwarul; Huq, Anwar; Colwell, Rita R.; Sack, R. Bradley; Stine, O. Colin.

In: Emerging Infectious Diseases, Vol. 20, No. 1, 2014, p. 54-60.

Research output: Contribution to journalArticle

Rashed, SM, Azman, AS, Alam, M, Li, S, Sack, DA, Morris, JG, Longini, I, Siddique, AK, Iqbal, A, Huq, A, Colwell, RR, Sack, RB & Stine, OC 2014, 'Genetic variation of vibrio cholerae during outbreaks, Bangladesh, 2010-2011', Emerging Infectious Diseases, vol. 20, no. 1, pp. 54-60. https://doi.org/10.3201/eid2001.130796
Rashed, Shah M. ; Azman, Andrew S. ; Alam, Munirul ; Li, Shan ; Sack, David A. ; Morris, J. Glenn ; Longini, Ira ; Siddique, Abul Kasem ; Iqbal, Anwarul ; Huq, Anwar ; Colwell, Rita R. ; Sack, R. Bradley ; Stine, O. Colin. / Genetic variation of vibrio cholerae during outbreaks, Bangladesh, 2010-2011. In: Emerging Infectious Diseases. 2014 ; Vol. 20, No. 1. pp. 54-60.
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