Genetic transfer of antimicrobial resistance and enterotoxigenicity among Escherichia coli strains

K. Wachsmuth, J. DeBoy, K. Birkness, David Allen Sack, J. Wells

Research output: Contribution to journalArticle

Abstract

To understand the role of enterotoxin (Ent) plasmids in epidemics of enterotoxigenic (ET) Escherichia coli diarrheas in the United States, we studied the genetics of Ent plasmids in relation to E. coli serotypes and R plasmids. Twenty-nine ET E. coli strains, including all epidemic isolates available at the Centers for Disease Control, Atlanta, Ga. (CDC), were assessed for the ability to transfer antimicrobial resistances (if present) by conjugations, to mobilize a nonconjugative R plasmid, and to cotransfer enterotoxigenicity with R determinants. Of the 12 ET E. coli strains isolated in the United States, 5 were able to transfer R plasmids; one strain cotransferred detectable enterotoxigenicity. Another four U.S. isolates were able to mobilize plasmid DNA, but no toxin production was detected in transconjugants. Of 17 resistant ET E. coli from South Asia, 13 were able to transfer R plasmids; 5 of those 13 cotransferred detectable Ent plasmids. In all, 22 ET E. coli strains (76%) were able to initiate conjugation and genetic transfers. Six of these strains (20%) were able to cotransfer enterotoxigenicity with a conjugative R plasmid at a detectable frequency. One of the six strains transferred R and Ent determinants on a single plasmid. These data are addressed in relation to the observed immobility of Ent and R during outbreaks of ET E. coli, the efficacy of prophylactic tetracycline, and the worldwide occurrence of a limited number of ET E. coli serotypes.

Original languageEnglish (US)
Pages (from-to)278-283
Number of pages6
JournalAntimicrobial Agents and Chemotherapy
Volume23
Issue number2
StatePublished - 1983
Externally publishedYes

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Enterotoxigenic Escherichia coli
R Factors
Enterotoxins
Escherichia coli
Plasmids
Centers for Disease Control and Prevention (U.S.)
Genetic Conjugation
Tetracycline
Disease Outbreaks
Diarrhea
DNA

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Genetic transfer of antimicrobial resistance and enterotoxigenicity among Escherichia coli strains. / Wachsmuth, K.; DeBoy, J.; Birkness, K.; Sack, David Allen; Wells, J.

In: Antimicrobial Agents and Chemotherapy, Vol. 23, No. 2, 1983, p. 278-283.

Research output: Contribution to journalArticle

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AU - Wells, J.

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