Genetic relationship between Cryptococcus neoformans var. neoformans strains of serotypes A and D

Sarah P. Franzot, Bettina C. Fries, Wendy Cleare, Arturo Casadevall

Research output: Contribution to journalArticle

Abstract

Cryptococcus neoformans serotypes A and D are responsible for the overwhelming majority of infections in patients with AIDS. The genetic relationship between the serotypes is poorly understood, but there are significant differences in the epidemiology and clinical presentation of serotype A and D infections. We evaluated the genetic relationship between reference C. neoformans strains belonging to serotypes A and D by analyzing their URA5 sequences and restriction fragment length polymorphisms (RFLPs) with the C. neoformans repetitive element 1 (CNRE-1) probe. The results were compared to those previously obtained for isolates from Brazil and New York City by the same typing methods, and dendrograms were generated. Serotype A and D strains produced distinct RFLP patterns consistent with their separation into two major clusters in the dendrogram generated on the basis of RFLP data. Similarly, serotype A and D strains clustered independently on the basis of the nucleotide sequences of their URA5 genes. Pairwise comparisons revealed average numbers of nucleotide differences within serotypes A and D of 3.0 ± 1.7 and 7.2 ± 3.4, respectively (P <0.0001), and between serotypes A and D of 41.9 ± 2.7. In summary, our results indicate phylogenetic differences between the two serotypes of C. neoformans var. neoformans and suggest that these serotypes could probably be considered different varieties of C. neoformans.

Original languageEnglish (US)
Pages (from-to)2200-2204
Number of pages5
JournalJournal of Clinical Microbiology
Volume36
Issue number8
StatePublished - Aug 1998
Externally publishedYes

Fingerprint

Cryptococcus neoformans
Restriction Fragment Length Polymorphisms
Serogroup
Infection
Brazil
Acquired Immunodeficiency Syndrome
Epidemiology
Nucleotides

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Genetic relationship between Cryptococcus neoformans var. neoformans strains of serotypes A and D. / Franzot, Sarah P.; Fries, Bettina C.; Cleare, Wendy; Casadevall, Arturo.

In: Journal of Clinical Microbiology, Vol. 36, No. 8, 08.1998, p. 2200-2204.

Research output: Contribution to journalArticle

Franzot, Sarah P. ; Fries, Bettina C. ; Cleare, Wendy ; Casadevall, Arturo. / Genetic relationship between Cryptococcus neoformans var. neoformans strains of serotypes A and D. In: Journal of Clinical Microbiology. 1998 ; Vol. 36, No. 8. pp. 2200-2204.
@article{3e90466e7561428b8590b11340021cb2,
title = "Genetic relationship between Cryptococcus neoformans var. neoformans strains of serotypes A and D",
abstract = "Cryptococcus neoformans serotypes A and D are responsible for the overwhelming majority of infections in patients with AIDS. The genetic relationship between the serotypes is poorly understood, but there are significant differences in the epidemiology and clinical presentation of serotype A and D infections. We evaluated the genetic relationship between reference C. neoformans strains belonging to serotypes A and D by analyzing their URA5 sequences and restriction fragment length polymorphisms (RFLPs) with the C. neoformans repetitive element 1 (CNRE-1) probe. The results were compared to those previously obtained for isolates from Brazil and New York City by the same typing methods, and dendrograms were generated. Serotype A and D strains produced distinct RFLP patterns consistent with their separation into two major clusters in the dendrogram generated on the basis of RFLP data. Similarly, serotype A and D strains clustered independently on the basis of the nucleotide sequences of their URA5 genes. Pairwise comparisons revealed average numbers of nucleotide differences within serotypes A and D of 3.0 ± 1.7 and 7.2 ± 3.4, respectively (P <0.0001), and between serotypes A and D of 41.9 ± 2.7. In summary, our results indicate phylogenetic differences between the two serotypes of C. neoformans var. neoformans and suggest that these serotypes could probably be considered different varieties of C. neoformans.",
author = "Franzot, {Sarah P.} and Fries, {Bettina C.} and Wendy Cleare and Arturo Casadevall",
year = "1998",
month = "8",
language = "English (US)",
volume = "36",
pages = "2200--2204",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "8",

}

TY - JOUR

T1 - Genetic relationship between Cryptococcus neoformans var. neoformans strains of serotypes A and D

AU - Franzot, Sarah P.

AU - Fries, Bettina C.

AU - Cleare, Wendy

AU - Casadevall, Arturo

PY - 1998/8

Y1 - 1998/8

N2 - Cryptococcus neoformans serotypes A and D are responsible for the overwhelming majority of infections in patients with AIDS. The genetic relationship between the serotypes is poorly understood, but there are significant differences in the epidemiology and clinical presentation of serotype A and D infections. We evaluated the genetic relationship between reference C. neoformans strains belonging to serotypes A and D by analyzing their URA5 sequences and restriction fragment length polymorphisms (RFLPs) with the C. neoformans repetitive element 1 (CNRE-1) probe. The results were compared to those previously obtained for isolates from Brazil and New York City by the same typing methods, and dendrograms were generated. Serotype A and D strains produced distinct RFLP patterns consistent with their separation into two major clusters in the dendrogram generated on the basis of RFLP data. Similarly, serotype A and D strains clustered independently on the basis of the nucleotide sequences of their URA5 genes. Pairwise comparisons revealed average numbers of nucleotide differences within serotypes A and D of 3.0 ± 1.7 and 7.2 ± 3.4, respectively (P <0.0001), and between serotypes A and D of 41.9 ± 2.7. In summary, our results indicate phylogenetic differences between the two serotypes of C. neoformans var. neoformans and suggest that these serotypes could probably be considered different varieties of C. neoformans.

AB - Cryptococcus neoformans serotypes A and D are responsible for the overwhelming majority of infections in patients with AIDS. The genetic relationship between the serotypes is poorly understood, but there are significant differences in the epidemiology and clinical presentation of serotype A and D infections. We evaluated the genetic relationship between reference C. neoformans strains belonging to serotypes A and D by analyzing their URA5 sequences and restriction fragment length polymorphisms (RFLPs) with the C. neoformans repetitive element 1 (CNRE-1) probe. The results were compared to those previously obtained for isolates from Brazil and New York City by the same typing methods, and dendrograms were generated. Serotype A and D strains produced distinct RFLP patterns consistent with their separation into two major clusters in the dendrogram generated on the basis of RFLP data. Similarly, serotype A and D strains clustered independently on the basis of the nucleotide sequences of their URA5 genes. Pairwise comparisons revealed average numbers of nucleotide differences within serotypes A and D of 3.0 ± 1.7 and 7.2 ± 3.4, respectively (P <0.0001), and between serotypes A and D of 41.9 ± 2.7. In summary, our results indicate phylogenetic differences between the two serotypes of C. neoformans var. neoformans and suggest that these serotypes could probably be considered different varieties of C. neoformans.

UR - http://www.scopus.com/inward/record.url?scp=0031927833&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031927833&partnerID=8YFLogxK

M3 - Article

VL - 36

SP - 2200

EP - 2204

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 8

ER -