Genetic profiling by single-nucleotide polymorphism-based array analysis defines three distinct subtypes of orbital meningioma

Cheng Ying Ho, Stacy Mosier, Janice Safneck, Diva R. Salomao, Neil R Miller, Charles G Eberhart, Christopher Gocke, Denise Batista, Fausto J Rodriguez

Research output: Contribution to journalArticle

Abstract

Orbital meningiomas can be classified as primary optic nerve sheath (ON) meningiomas, primary intraorbital ectopic (Ob) meningiomas and spheno-orbital (Sph-Ob) meningiomas based on anatomic site. Single-nucleotide polymorphism (SNP)-based array analysis with the Illumina 300K platform was performed on formalin-fixed, paraffin-embedded tissue from 19 orbital meningiomas (5 ON, 4 Ob and 10 Sph-Ob meningiomas). Tumors were World Health Organization (WHO) grade I except for two grade II meningiomas, and one was NF2-associated. We found genomic alterations in 68% (13 of 19) of orbital meningiomas. Sph-Ob tumors frequently exhibited monosomy 22/22q loss (70%; 7/10) and deletion of chromosome 1p, 6q and 19p (50% each; 5/10). Among genetic alterations, loss of chromosome 1p and 6q were more frequent in clinically progressive tumors. Chromosome 22q loss also was detected in the majority of Ob meningiomas (75%; 3/4) but was infrequent in ON meningiomas (20%; 1/5). In general, Ob tumors had fewer chromosome alterations than Sph-Ob and ON tumors. Unlike Sph-Ob meningiomas, most of the Ob and ON meningiomas did not progress even after incomplete excision, although follow-up was limited in some cases. Our study suggests that ON, Ob and Sph-Ob meningiomas are three molecularly distinct entities. Our results also suggest that molecular subclassification may have prognostic implications.

Original languageEnglish (US)
Pages (from-to)193-201
Number of pages9
JournalBrain Pathology
Volume25
Issue number2
DOIs
StatePublished - Mar 1 2015

Fingerprint

Meningioma
Single Nucleotide Polymorphism
Optic Nerve
Chromosomes
Optic Nerve Neoplasms
Neoplasms
Monosomy
Chromosomes, Human, Pair 10
Paraffin
Formaldehyde

Keywords

  • chromosome 22
  • cytogenetics
  • NF2
  • optic nerve sheath
  • orbital meningioma
  • SNP array

ASJC Scopus subject areas

  • Neuroscience(all)
  • Pathology and Forensic Medicine
  • Clinical Neurology

Cite this

Genetic profiling by single-nucleotide polymorphism-based array analysis defines three distinct subtypes of orbital meningioma. / Ho, Cheng Ying; Mosier, Stacy; Safneck, Janice; Salomao, Diva R.; Miller, Neil R; Eberhart, Charles G; Gocke, Christopher; Batista, Denise; Rodriguez, Fausto J.

In: Brain Pathology, Vol. 25, No. 2, 01.03.2015, p. 193-201.

Research output: Contribution to journalArticle

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abstract = "Orbital meningiomas can be classified as primary optic nerve sheath (ON) meningiomas, primary intraorbital ectopic (Ob) meningiomas and spheno-orbital (Sph-Ob) meningiomas based on anatomic site. Single-nucleotide polymorphism (SNP)-based array analysis with the Illumina 300K platform was performed on formalin-fixed, paraffin-embedded tissue from 19 orbital meningiomas (5 ON, 4 Ob and 10 Sph-Ob meningiomas). Tumors were World Health Organization (WHO) grade I except for two grade II meningiomas, and one was NF2-associated. We found genomic alterations in 68{\%} (13 of 19) of orbital meningiomas. Sph-Ob tumors frequently exhibited monosomy 22/22q loss (70{\%}; 7/10) and deletion of chromosome 1p, 6q and 19p (50{\%} each; 5/10). Among genetic alterations, loss of chromosome 1p and 6q were more frequent in clinically progressive tumors. Chromosome 22q loss also was detected in the majority of Ob meningiomas (75{\%}; 3/4) but was infrequent in ON meningiomas (20{\%}; 1/5). In general, Ob tumors had fewer chromosome alterations than Sph-Ob and ON tumors. Unlike Sph-Ob meningiomas, most of the Ob and ON meningiomas did not progress even after incomplete excision, although follow-up was limited in some cases. Our study suggests that ON, Ob and Sph-Ob meningiomas are three molecularly distinct entities. Our results also suggest that molecular subclassification may have prognostic implications.",
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