Genetic organization and sequence analysis of the hypha-specific cell wall protein gene HWP1 of Candida albicans

Janet F. Staab, Paula Sundstrom

Research output: Contribution to journalArticlepeer-review

Abstract

A previously isolated partial cDNA encoding a cell wall protein antigen found on hyphal surfaces of the opportunistic fungal pathogen, Candida albicans (Staab et al., 1996) was used to clone the complete hyphal wall protein 1 gene (HWP1). Hyphal forms of C. albicans invade mucosal surfaces of immunocompromised patients such as those with AIDS. HWP1 consisted of an open reading frame predicting an acidic protein (pI of 3.37) with a calculated molecular size of 61,122. The antigenic domain was located in the N-terminal third of the protein. The remainder of the protein contained abundant hydroxy amino acids, and terminated with a string of 15 amino acids typical of sequences specifying post-translational modification with glycosylphosphatidylinositol (6PI). The analyses suggested that Hwp1 is a glucan-linked protein with serine/threonine-rich regions that are predicted to function in extending a ligand-binding domain into the extracellular space. The nucleotide sequence reported in this paper has been submitted to GenBank/EMBL databank with Accession Number U64206.

Original languageEnglish (US)
Pages (from-to)681-686
Number of pages6
JournalYeast
Volume14
Issue number7
DOIs
StatePublished - May 1998

Keywords

  • Candida albicnns
  • Cell wall protein
  • DNA sequence
  • Glutamine-rich
  • HWP1
  • Hypha-specific
  • Proline-rich
  • RACE
  • Serine and thrconinc-rich

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Genetics

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