Genetic mosaic analysis of stem cell lineages in the Drosophila ovary

Kaitlin M. Laws, Daniela Drummond-Barbosa

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Genetic mosaic analyses represent an invaluable approach for the study of stem cell lineages in the Drosophila ovary. The generation of readily identifiable, homozygous mutant cells in the context of wild- type ovarian tissues within intact organisms allows the pinpointing of cellular requirements for gene function, which is particularly important for understanding the physiological control of stem cells and their progeny. Here, we provide a step-by-step guide to the generation and analysis of genetically mosaic ovaries using flippase (FLP)/ FLP recognition target ( FRT )-mediated recombination in adult Drosophila melanogaster, with a focus on the processes of oogenesis that are controlled by diet-dependent factors.

Original languageEnglish (US)
Title of host publicationDrosophila Oogenesis
Subtitle of host publicationMethods and Protocols
PublisherSpringer New York
Pages57-72
Number of pages16
ISBN (Electronic)9781493928514
ISBN (Print)9781493928507
DOIs
StatePublished - Aug 31 2015

Keywords

  • Clonal analysis
  • Drosophila
  • FLP/FRT
  • Follicle cell
  • Follicle stem cell
  • Genetic mosaics
  • Germline stem cell
  • Oogenesis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Medicine(all)

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