Abstract
Mutational analyses have shown that the genes no tail (nt1, Brachyury homolog), floating head (flh, a Not homeobox gene), and cyclops (cyc) play direct and essential roles in the development of midline structures in the zebrafish. In both nt1 and flh mutants a notochord does not develop, and in cyc mutants the floor plate is nearly entirely missing. We made double mutants to learn how these genes might interact. Midline development is disrupted to a greater extent in cyc;flh double mutants than in either cyc or flh single mutants; their effects appear additive. Both the notochord and floor plate are completely lacking, and other phenotypic disturbances suggest that midline signaling functions are severely reduced. On the other hand, trunk midline defects in flh;nt1 double mutants are not additive, but are most often similar to those in nt1 single mutants. This finding reveals that loss of nt1 function can suppress phenotypic defects due to mutation at flh, and we interpret it to mean that the wild-type allele of nt1 (nt1+) functions upstream to flh in a regulatory hierarchy. Loss of function of nt1 also strongly suppresses the floor plate deficiency in cyc mutants, for we found trunk floor plate to be present in cyc;nt1 double mutants. From these findings we propose that nt1+ plays an early role in cell fate choice at the dorsal midline, mediated by the Nt1 protein acting to antagonize floor plate development as well as to promote notochord development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 154-170 |
| Number of pages | 17 |
| Journal | Developmental Biology |
| Volume | 187 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jul 15 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine(all)
- Molecular Biology
- Developmental Biology
- Cell Biology
Cite this
Genetic interactions in zebrafish midline development. / Halpern, Marnie; Hatta, Kohei; Amacher, Sharon L.; Talbot, William S.; Yan, Yi Lin; Thisse, Bernard; Thisse, Christine; Postlethwait, John H.; Kimmel, Charles B.
In: Developmental Biology, Vol. 187, No. 2, 15.07.1997, p. 154-170.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genetic interactions in zebrafish midline development
AU - Halpern, Marnie
AU - Hatta, Kohei
AU - Amacher, Sharon L.
AU - Talbot, William S.
AU - Yan, Yi Lin
AU - Thisse, Bernard
AU - Thisse, Christine
AU - Postlethwait, John H.
AU - Kimmel, Charles B.
PY - 1997/7/15
Y1 - 1997/7/15
N2 - Mutational analyses have shown that the genes no tail (nt1, Brachyury homolog), floating head (flh, a Not homeobox gene), and cyclops (cyc) play direct and essential roles in the development of midline structures in the zebrafish. In both nt1 and flh mutants a notochord does not develop, and in cyc mutants the floor plate is nearly entirely missing. We made double mutants to learn how these genes might interact. Midline development is disrupted to a greater extent in cyc;flh double mutants than in either cyc or flh single mutants; their effects appear additive. Both the notochord and floor plate are completely lacking, and other phenotypic disturbances suggest that midline signaling functions are severely reduced. On the other hand, trunk midline defects in flh;nt1 double mutants are not additive, but are most often similar to those in nt1 single mutants. This finding reveals that loss of nt1 function can suppress phenotypic defects due to mutation at flh, and we interpret it to mean that the wild-type allele of nt1 (nt1+) functions upstream to flh in a regulatory hierarchy. Loss of function of nt1 also strongly suppresses the floor plate deficiency in cyc mutants, for we found trunk floor plate to be present in cyc;nt1 double mutants. From these findings we propose that nt1+ plays an early role in cell fate choice at the dorsal midline, mediated by the Nt1 protein acting to antagonize floor plate development as well as to promote notochord development.
AB - Mutational analyses have shown that the genes no tail (nt1, Brachyury homolog), floating head (flh, a Not homeobox gene), and cyclops (cyc) play direct and essential roles in the development of midline structures in the zebrafish. In both nt1 and flh mutants a notochord does not develop, and in cyc mutants the floor plate is nearly entirely missing. We made double mutants to learn how these genes might interact. Midline development is disrupted to a greater extent in cyc;flh double mutants than in either cyc or flh single mutants; their effects appear additive. Both the notochord and floor plate are completely lacking, and other phenotypic disturbances suggest that midline signaling functions are severely reduced. On the other hand, trunk midline defects in flh;nt1 double mutants are not additive, but are most often similar to those in nt1 single mutants. This finding reveals that loss of nt1 function can suppress phenotypic defects due to mutation at flh, and we interpret it to mean that the wild-type allele of nt1 (nt1+) functions upstream to flh in a regulatory hierarchy. Loss of function of nt1 also strongly suppresses the floor plate deficiency in cyc mutants, for we found trunk floor plate to be present in cyc;nt1 double mutants. From these findings we propose that nt1+ plays an early role in cell fate choice at the dorsal midline, mediated by the Nt1 protein acting to antagonize floor plate development as well as to promote notochord development.
UR - http://www.scopus.com/inward/record.url?scp=0030804983&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030804983&partnerID=8YFLogxK
U2 - 10.1006/dbio.1997.8605
DO - 10.1006/dbio.1997.8605
M3 - Article
C2 - 9242414
AN - SCOPUS:0030804983
VL - 187
SP - 154
EP - 170
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 2
ER -