TY - JOUR
T1 - Genetic diversity of a population of Schistosoma haematobium derived from schoolchildren in East Central Zimbabwe
AU - Brouwer, K. C.
AU - Ndhlovu, P.
AU - Munatsi, A.
AU - Shiff, C. J.
PY - 2001
Y1 - 2001
N2 - To characterize the extent of genetic diversity of Schistosoma haematobium within and among its definitive host (intra- and interhost parasite diversity), 133 individual isolates from 25 infected schoolchildren were compared using randomly amplified polymorphic DNA markers. With 4 primers, 53 unambiguous loci were identified, and of these, 22 were polymorphic. Mean heterozygosity in the population was 0.116 ± 0.043. Analysis of molecular variance showed the majority of variance occurred within, rather than between, hosts. Frequencies of certain alleles segregated the parasite population into 13 distinct clusters of associated genotypes, with 4 of these first appearing 10 mo after the initial survey. Considering the level of diversity within this limited geographical area and the possibility of rapid turnover of genotypes, parasite variance may impact acquired immunity and clinical outcome of the infection.
AB - To characterize the extent of genetic diversity of Schistosoma haematobium within and among its definitive host (intra- and interhost parasite diversity), 133 individual isolates from 25 infected schoolchildren were compared using randomly amplified polymorphic DNA markers. With 4 primers, 53 unambiguous loci were identified, and of these, 22 were polymorphic. Mean heterozygosity in the population was 0.116 ± 0.043. Analysis of molecular variance showed the majority of variance occurred within, rather than between, hosts. Frequencies of certain alleles segregated the parasite population into 13 distinct clusters of associated genotypes, with 4 of these first appearing 10 mo after the initial survey. Considering the level of diversity within this limited geographical area and the possibility of rapid turnover of genotypes, parasite variance may impact acquired immunity and clinical outcome of the infection.
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U2 - 10.1645/0022-3395(2001)087[0762:gdoapo]2.0.co;2
DO - 10.1645/0022-3395(2001)087[0762:gdoapo]2.0.co;2
M3 - Article
C2 - 11534639
AN - SCOPUS:0034857196
SN - 0022-3395
VL - 87
SP - 762
EP - 769
JO - Journal of Parasitology
JF - Journal of Parasitology
IS - 4
ER -