Genetic disruption of PPARδ decreases the tumorigenicity of human colon cancer cells

Research output: Contribution to journalArticle

Abstract

Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors that have been implicated in a variety of biologic processes. The PPARδ isotype was recently proposed as a downstream target of the adenomatous polyposis coli (APC)/β-catenin pathway in colorectal carcinogenesis. To evaluate its role in tumorigenesis, a PPARδ null cell line was created by targeted homologous recombination. When inoculated as xenografts in nude mice, PPARδ -/- cells exhibited a decreased ability to form tumors compared with PPARδ +/- and wild-type controls. These data suggest that suppression of PPARδ expression contributes to the growth-inhibitory effects of the APC tumor suppressor.

Original languageEnglish (US)
Pages (from-to)2598-2603
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number5
DOIs
StatePublished - Feb 27 2001

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Peroxisome Proliferator-Activated Receptors
Colonic Neoplasms
Adenomatous Polyposis Coli
Carcinogenesis
Catenins
Null Lymphocytes
Homologous Recombination
Cytoplasmic and Nuclear Receptors
Heterografts
Nude Mice
Neoplasms
Cell Line
Growth

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

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abstract = "Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors that have been implicated in a variety of biologic processes. The PPARδ isotype was recently proposed as a downstream target of the adenomatous polyposis coli (APC)/β-catenin pathway in colorectal carcinogenesis. To evaluate its role in tumorigenesis, a PPARδ null cell line was created by targeted homologous recombination. When inoculated as xenografts in nude mice, PPARδ -/- cells exhibited a decreased ability to form tumors compared with PPARδ +/- and wild-type controls. These data suggest that suppression of PPARδ expression contributes to the growth-inhibitory effects of the APC tumor suppressor.",
author = "Park, {Ben Ho} and Bert Vogelstein and Kinzler, {Kenneth W}",
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AB - Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors that have been implicated in a variety of biologic processes. The PPARδ isotype was recently proposed as a downstream target of the adenomatous polyposis coli (APC)/β-catenin pathway in colorectal carcinogenesis. To evaluate its role in tumorigenesis, a PPARδ null cell line was created by targeted homologous recombination. When inoculated as xenografts in nude mice, PPARδ -/- cells exhibited a decreased ability to form tumors compared with PPARδ +/- and wild-type controls. These data suggest that suppression of PPARδ expression contributes to the growth-inhibitory effects of the APC tumor suppressor.

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