Genetic contribution to the septic response in a mouse model

Fray Dylan Stewart, William B. Fulton, Chad Wilson, Constance L Monitto, Charles N. Paidas, Roger H Reeves, Antonio De Maio

Research output: Contribution to journalArticle

Abstract

The response to injury is dependent on several factors, including the type and extent of the injury, genetics, and the environment. In the present study, the genetic contribution to sepsis was evaluated in a mouse model. Sepsis was induced in two inbred mouse strains, C57BL/6J (B6) and A/J, by cecal ligation and single puncture (CLP). Frequency of mortality was significantly higher in B6 than A/J mice from 36 to 132 h after CLP. Plasma TNF-α, IL-1β, and IL-6 levels were similar in both strains after CLP. IL-10 plasma levels were significantly higher in B6 mice as opposed to A/J mice after 24 h of CLP. Similarly, hepatic myeloperoxidase activity, an index of polymorphonuclear leukocytes, was elevated in B6 mice as compared with A/J mice after 24 h of CLP. On the contrary, metallothionein mRNA levels were higher in A/J mice compared with B6 mice. Finally, leptin levels were also higher in A/J than B6 mice within 19 h of CLP. This study demonstrates a genetic contribution in the response to sepsis.

Original languageEnglish (US)
Pages (from-to)342-347
Number of pages6
JournalShock
Volume18
Issue number4
StatePublished - Oct 2002

Fingerprint

Punctures
Ligation
Sepsis
Inbred Strains Mice
Metallothionein
Wounds and Injuries
Leptin
Interleukin-1
Interleukin-10
Peroxidase
Interleukin-6
Neutrophils
Messenger RNA
Mortality
Liver

Keywords

  • Acute phase
  • Cytokines
  • Genetics
  • Inflammation
  • Leptin
  • Sepsis

ASJC Scopus subject areas

  • Physiology
  • Critical Care and Intensive Care Medicine

Cite this

Stewart, F. D., Fulton, W. B., Wilson, C., Monitto, C. L., Paidas, C. N., Reeves, R. H., & De Maio, A. (2002). Genetic contribution to the septic response in a mouse model. Shock, 18(4), 342-347.

Genetic contribution to the septic response in a mouse model. / Stewart, Fray Dylan; Fulton, William B.; Wilson, Chad; Monitto, Constance L; Paidas, Charles N.; Reeves, Roger H; De Maio, Antonio.

In: Shock, Vol. 18, No. 4, 10.2002, p. 342-347.

Research output: Contribution to journalArticle

Stewart, FD, Fulton, WB, Wilson, C, Monitto, CL, Paidas, CN, Reeves, RH & De Maio, A 2002, 'Genetic contribution to the septic response in a mouse model', Shock, vol. 18, no. 4, pp. 342-347.
Stewart FD, Fulton WB, Wilson C, Monitto CL, Paidas CN, Reeves RH et al. Genetic contribution to the septic response in a mouse model. Shock. 2002 Oct;18(4):342-347.
Stewart, Fray Dylan ; Fulton, William B. ; Wilson, Chad ; Monitto, Constance L ; Paidas, Charles N. ; Reeves, Roger H ; De Maio, Antonio. / Genetic contribution to the septic response in a mouse model. In: Shock. 2002 ; Vol. 18, No. 4. pp. 342-347.
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