TY - JOUR
T1 - Genetic characterization of immortalized human prostate epithelial cell cultures
T2 - Evidence for structural rearrangements of chromosome 8 and i(8q) chromosome formation in primary tumor-derived cells
AU - Macoska, Jill A.
AU - Beheshti, Ben
AU - Rhim, Johng S.
AU - Hukku, Bharati
AU - Lehr, Jeff
AU - Pienta, Kenneth J.
AU - Squire, Jeremy A.
N1 - Funding Information:
The authors would like to thank Ms. Tanya Trybus and Ms. Jana Karaskova for their technical contributions to these studies. We would also like to thank Dr. Suzanne Topalian for her kind gift of the HPV-immortalized cell lines. This work was supported by grants R29 CA60948 from the National Institutes of Health, and RPG-98-338-01-MGO from The American Cancer Society (J. A. M.), the NIH/NCI 1P50 CA69568 SPORE in Prostate Cancer (K. J. P.), and by U.S. Army Medical Research and Materiel Command (USAMRMC) Prostate Cancer Research Program (PCRP) Grant PC970601 (J. A. S.)
PY - 2000/7/1
Y1 - 2000/7/1
N2 - We have utilized a combination of conventional and spectral karyotyping (SKY) techniques and allelotype analysis to assess numerical and structural chromosome alterations in two cell lines derived from normal human prostatic epithelium, and three cell lines derived from human prostate primary tumor epithelium, immortalized with the E6 and E7 transforming genes of human papilloma virus (HPV) 16 or the large T-antigen gene of simian virus 40 (SV40). These studies revealed trisomy for chromosome 20 and rearrangements involving chromosomes 3, 4, 8, 9, 10, 16, 17, 18, 19, 21, or 22. In addition, the four HPV-immortalized cell lines exhibited extensive duplications or translocations involving the 11q chromosomal region. Interestingly, allelotyping data disclosed loss of 8p sequences in two of the three primary tumor-derived cell lines, and SKY data revealed that the loss of 8p sequences was directly due to i(8q) chromosome formation and/or other structural alterations of chromosome 8. This provides intriguing evidence that 8p loss in primary human prostate tumors may, in some cases, result from complex structural rearrangements involving chromosome 8. Moreover, the data reported here provide direct evidence that such complex structural rearrangements sometimes include i(8q) chromosome formation. Copyright (C) 2000 Elsevier Science Inc.
AB - We have utilized a combination of conventional and spectral karyotyping (SKY) techniques and allelotype analysis to assess numerical and structural chromosome alterations in two cell lines derived from normal human prostatic epithelium, and three cell lines derived from human prostate primary tumor epithelium, immortalized with the E6 and E7 transforming genes of human papilloma virus (HPV) 16 or the large T-antigen gene of simian virus 40 (SV40). These studies revealed trisomy for chromosome 20 and rearrangements involving chromosomes 3, 4, 8, 9, 10, 16, 17, 18, 19, 21, or 22. In addition, the four HPV-immortalized cell lines exhibited extensive duplications or translocations involving the 11q chromosomal region. Interestingly, allelotyping data disclosed loss of 8p sequences in two of the three primary tumor-derived cell lines, and SKY data revealed that the loss of 8p sequences was directly due to i(8q) chromosome formation and/or other structural alterations of chromosome 8. This provides intriguing evidence that 8p loss in primary human prostate tumors may, in some cases, result from complex structural rearrangements involving chromosome 8. Moreover, the data reported here provide direct evidence that such complex structural rearrangements sometimes include i(8q) chromosome formation. Copyright (C) 2000 Elsevier Science Inc.
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U2 - 10.1016/S0165-4608(99)00248-4
DO - 10.1016/S0165-4608(99)00248-4
M3 - Article
C2 - 10913677
AN - SCOPUS:0034237346
SN - 0165-4608
VL - 120
SP - 50
EP - 57
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
IS - 1
ER -