Genetic architecture of HIV Type 1 Nef and Tat from HLA-B57-typed long-term survivors in an indian cohort of perinatally HIV-infected children

Ujjwal Neogi, Riya Palchaudhuri, Sankhya Bommana, Anita Shet

Research output: Contribution to journalArticlepeer-review

Abstract

Human immunodeficiency virus type 1 (HIV-1) viral genes nef and tat play an important role in disease progression. In this study we characterized the Nef and Tat proteins from a group of HLA-B57 typed pediatric perinatally infected long-term survivors (LTS) with ≥10 years of infection. We identified 19 therapy-naive LTS after screening 250 children from an Indian pediatric cohort. Nef and tat amplified from plasma virus showed that all the LTS harbored HIV-1 subtype C. The two B57+ children showed mutations, deletions, and insertions in experimentally defined B57 epitopes in the virus that are likely to be escape mutants. Only GW12 (GPGVRYPLTFGW) and YY9 (YTPGPGIRY) were conserved, while the remaining 90% (18/20) of the epitopes showed some degree of mutations. The most variable epitopes were RR15, SE15, QP15, KF9, HW9, YT9, and GF15. To our knowledge this is the first study from India in which characterization of Nef and Tat from LTS has led to information on genetic alterations in these genes that are associated with slow disease progression, and can provide an important lead in future studies.

Original languageEnglish (US)
Pages (from-to)1613-1616
Number of pages4
JournalAIDS research and human retroviruses
Volume29
Issue number12
DOIs
StatePublished - Dec 1 2013
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Genetic architecture of HIV Type 1 Nef and Tat from HLA-B57-typed long-term survivors in an indian cohort of perinatally HIV-infected children'. Together they form a unique fingerprint.

Cite this