Genetic, antigenic and serologic characterization of human immunodeficiency virus type 1 from Indonesia

Kevin R. Porter, John R. Mascola, Hudoyo Hupudio, Daniel Ewing, Thomas C. VanCott, Ronald L. Anthony, Andrew L. Corwin, Soeprapto Widodo, Soekardjo Ertono, Francine E. McCutchan, Donald S. Burke, Curtis G. Hayes, F. Stephen Wignall, Robert R. Graham

Research output: Contribution to journalArticle

Abstract

To examine the genetic and antigenic characteristics of HIV-1 in Indonesia, samples from 19 HIV-positive volunteers were studied. By a combination of PCR typing and DNA sequence analysis, 12 of the 19 volunteers were determined to be infected with HIV-1 clade B and seven with clade E. Six of the seven Indonesian clade E isolates were from volunteers associated with the Indonesian Military during a peacekeeping mission in Cambodia. Infectivity reduction neutralization assays showed that the Indonesian E viruses were effectively neutralized by Thailand clade E HIV-1 antisera but not by U.S. clade B antisera. The Indonesian clade B virus tested was neutralized by U.S. clade B antisera and not by the Thailand E antisera. Using a previously described serologic typing ELISA based on clade B and E V3 peptides, genetic clade was accurately determined in eight of eight sera tested. This is the first report of the genetic and antigenic analysis of HIV-1 isolates from Indonesia. The data indicate that at least two genetic and antigenic HIV-1 clades (clade E and B) circulate in Indonesia.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalJournal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Volume14
Issue number1
DOIs
StatePublished - Jan 1 1997

Keywords

  • Genotype
  • HIV-1
  • Neutralization tests

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Virology

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    Porter, K. R., Mascola, J. R., Hupudio, H., Ewing, D., VanCott, T. C., Anthony, R. L., Corwin, A. L., Widodo, S., Ertono, S., McCutchan, F. E., Burke, D. S., Hayes, C. G., Wignall, F. S., & Graham, R. R. (1997). Genetic, antigenic and serologic characterization of human immunodeficiency virus type 1 from Indonesia. Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, 14(1), 1-6. https://doi.org/10.1097/00042560-199701010-00001