Genetic and phenotypic analysis of flagellar assembly mutants in Chlamydomonas reinhardtii.

Carlo Iomini; Jacob E. Till; Susan K. Dutcher

doi:10.1016/S0091-679X(08)93007-7

Genetic and phenotypic analysis of flagellar assembly mutants in Chlamydomonas reinhardtii.

Carlo Iomini, Jacob E. Till, Susan K. Dutcher

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Conditional mutants for flagellar assembly (fla) provide a useful tool to study intraflagellar transport (IFT) at the molecular level, and provide a unique set of tools to analyze cilia. The analysis of IFT phenotypes of fla mutants at the permissive temperature by a quantitative image analysis approach identified four distinct phases of the IFT cycle and directly demonstrated structural and functional remodeling of IFT particles at both axonemal extremities. In addition, the genetic analysis of fla mutants reveal interesting interactions among genes involved in flagellar assembly that help to provide information about the structure and function of IFT particles and their motors. This chapter provides protocols to isolate, characterize, and identify conditional Chlamydomonas flagellar assembly mutants and their genes and to test genetic interactions among proteins encoded by these genes. 2009 Elsevier Inc. All rights reserved.

Original language	English (US)
Pages (from-to)	121-143
Number of pages	23
Journal	Methods in cell biology
Volume	93
DOIs	https://doi.org/10.1016/S0091-679X(08)93007-7
State	Published - 2009
Externally published	Yes

ASJC Scopus subject areas

Cell Biology

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10.1016/S0091-679X(08)93007-7

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title = "Genetic and phenotypic analysis of flagellar assembly mutants in Chlamydomonas reinhardtii.",

abstract = "Conditional mutants for flagellar assembly (fla) provide a useful tool to study intraflagellar transport (IFT) at the molecular level, and provide a unique set of tools to analyze cilia. The analysis of IFT phenotypes of fla mutants at the permissive temperature by a quantitative image analysis approach identified four distinct phases of the IFT cycle and directly demonstrated structural and functional remodeling of IFT particles at both axonemal extremities. In addition, the genetic analysis of fla mutants reveal interesting interactions among genes involved in flagellar assembly that help to provide information about the structure and function of IFT particles and their motors. This chapter provides protocols to isolate, characterize, and identify conditional Chlamydomonas flagellar assembly mutants and their genes and to test genetic interactions among proteins encoded by these genes. 2009 Elsevier Inc. All rights reserved.",

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AU - Till, Jacob E.

AU - Dutcher, Susan K.

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AB - Conditional mutants for flagellar assembly (fla) provide a useful tool to study intraflagellar transport (IFT) at the molecular level, and provide a unique set of tools to analyze cilia. The analysis of IFT phenotypes of fla mutants at the permissive temperature by a quantitative image analysis approach identified four distinct phases of the IFT cycle and directly demonstrated structural and functional remodeling of IFT particles at both axonemal extremities. In addition, the genetic analysis of fla mutants reveal interesting interactions among genes involved in flagellar assembly that help to provide information about the structure and function of IFT particles and their motors. This chapter provides protocols to isolate, characterize, and identify conditional Chlamydomonas flagellar assembly mutants and their genes and to test genetic interactions among proteins encoded by these genes. 2009 Elsevier Inc. All rights reserved.

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Genetic and phenotypic analysis of flagellar assembly mutants in Chlamydomonas reinhardtii.

Abstract

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