Genetic and environmental effects on glucosinolate content and chemoprotective potency of broccoli

M. W. Farnham, P. E. Wilson, K. K. Stephenson, J. W. Fahey

Research output: Contribution to journalArticle

Abstract

Broccoli is well recognized as a source of glucosinolates and their isothiocyanate breakdown products. Glucoraphanin is one of the most abundant glucosinolates present in broccoli and its cognate isothiocyanate is sulphoraphane, a potent inducer of mammalian detoxication (phase 2) enzyme activity and anti-cancer agent. This study was designed to measure: glucosinolate levels in broccoli florets from an array of genotypes grown in several environments; the elevation of a key phase 2 enzyme, quinone reductase, in mammalian cells exposed to floret extracts; and total broccoli head content. There were significant environmental and genotype-by-environment effects on levels of glucoraphanin and quinone reductase induction potential of broccoli heads; however, the effect of genotype was greater than that of environmental factors. The relative rankings among genotypes for glucoraphanin and quinone reductase induction potential changed, when expressed on a per head basis, rather than on a concentration basis. Correlations of trait means in one environment vs. means from a second were stronger for glucoraphanin and quinone reductase induction potential on a per head basis than on a fresh weight concentration basis. Results of this study indicate that development of a broccoli phenotype with a dense head and a high concentration of glucoraphanin to deliver maximum chemoprotective potential (high enzyme induction potential/glucoraphanin content) is a feasible goal.

Original languageEnglish (US)
Pages (from-to)60-65
Number of pages6
JournalPlant Breeding
Volume123
Issue number1
DOIs
StatePublished - Feb 1 2004

Keywords

  • Brassica oleracea
  • Doubled haploids
  • Glucoraphanin
  • Isothiocyanates
  • Italica group
  • Nutrition
  • Sulphoraphane

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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