Genetic analysis of superoxide dismutase, the 23 kilodalton antigen of Mycobacterium tuberculosis

Ying Zhang, R. Lathigra, T. Garbe, D. Catty, D. Young

Research output: Contribution to journalArticle

Abstract

The gene encoding a 23 kilodalton protein antigen has been cloned from Mycobacterium tuberculosis by screening of a recombinant DNA library with monoclonal antibodies. The product of the gene has been identified as the superoxide dismutase (SOD) of M. tuberculosis on the basis of sequence comparison and by expression of the recombinant protein in a functionally active form. The derived amino acid sequence of M. tuberculosis SOD reveals a close similarity to manganese-containing SODs from other organisms, in spite of the fact that previous studies using the purified enzyme have identified iron as the preferred metal ion ligand. SOD is present in the extracellular fluid of logarithmic-phase cultures of M. tuberculosis, but the structural gene is not preceded by a signal peptide sequence. Insertion of the M. tuberculosis SOD gene into a novel shuttle vector demonstrated the presence of a promoter which functions efficiently in mycobacteria but is ineffective in Escherichia coli.

Original languageEnglish (US)
Pages (from-to)381-391
Number of pages11
JournalMolecular Microbiology
Volume5
Issue number2
StatePublished - Feb 1991
Externally publishedYes

Fingerprint

Mycobacterium tuberculosis
Superoxide Dismutase
Genes
Genetic Vectors
Recombinant DNA
Extracellular Fluid
Mycobacterium
Manganese
Protein Sorting Signals
Gene Library
Recombinant Proteins
Amino Acid Sequence
Iron
Metals
Monoclonal Antibodies
Mycobacterium tuberculosis antigens
Ions
Escherichia coli
Ligands
Antigens

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Genetic analysis of superoxide dismutase, the 23 kilodalton antigen of Mycobacterium tuberculosis. / Zhang, Ying; Lathigra, R.; Garbe, T.; Catty, D.; Young, D.

In: Molecular Microbiology, Vol. 5, No. 2, 02.1991, p. 381-391.

Research output: Contribution to journalArticle

Zhang, Ying ; Lathigra, R. ; Garbe, T. ; Catty, D. ; Young, D. / Genetic analysis of superoxide dismutase, the 23 kilodalton antigen of Mycobacterium tuberculosis. In: Molecular Microbiology. 1991 ; Vol. 5, No. 2. pp. 381-391.
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