Genes expressed in Brugia malayi infective third stage larvae

Mark L. Blaxter, Nithyakalyani Raghavan, Inca Ghosh, David Guiliano, Wenhong Lu, Steven A. Williams, Barton Slatko, Alan L. Scott

Research output: Contribution to journalArticle

Abstract

We have used a tag sequencing approach to survey genes expressed in the third stage infective larvae of the human filarial nematode parasite Brugia malayi. RNA was isolated from late vector-stage L3 larvae after days 9 or 10 of infection in mosquitos, and converted to cDNA by reverse transcriptase. Double-stranded cDNA was produced by either conventional methods (non-SL cDNA library) or by PCR using the nematode spliced leader (SL1) and oligo(dT) primers (SL cDNA library). Two clone libraries (one from SL and one from non-SL cDNAs) were constructed in lambda ZapII. A set of these full-length clones was selected and 596 inserts were sequenced from the 5' end. We have identified 364 malayi genes (the majority of which are new) that encode housekeeping proteins, structural proteins, proteins of immediate immunological or drug-discovery interest as well as a large class of novel sequences which may prove to have significant involvement in host invasion. Extensive, genome-wide approaches to the analysis of larval gene expression are now possible for B. malayi. We present several examples of this approach.

Original languageEnglish (US)
Pages (from-to)77-93
Number of pages17
JournalMolecular and Biochemical Parasitology
Volume77
Issue number1
DOIs
StatePublished - Apr 1996

Keywords

  • Brugia malayi, third stage larva
  • Expressed sequence tag
  • Nematode
  • Polymerase chain reaction
  • Spliced leader
  • Trans-splicing

ASJC Scopus subject areas

  • Parasitology
  • Molecular Biology

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  • Cite this

    Blaxter, M. L., Raghavan, N., Ghosh, I., Guiliano, D., Lu, W., Williams, S. A., Slatko, B., & Scott, A. L. (1996). Genes expressed in Brugia malayi infective third stage larvae. Molecular and Biochemical Parasitology, 77(1), 77-93. https://doi.org/10.1016/0166-6851(96)02571-6