Generation of three-dimensional retinal tissue with functional photoreceptors from human iPSCs

Xiufeng Zhong; Christian Gutierrez; Tian Xue; Christopher Hampton; M. Natalia Vergara; Li Hui Cao; Ann Peters; Tea Soon Park; Elias Zambidis; Jason S. Meyer; David M. Gamm; King-Wai Yau; M. Valeria Canto-Soler

doi:10.1038/ncomms5047

Generation of three-dimensional retinal tissue with functional photoreceptors from human iPSCs

Xiufeng Zhong, Christian Gutierrez, Tian Xue, Christopher Hampton, M. Natalia Vergara, Li Hui Cao, Ann Peters, Tea Soon Park, Elias Zambidis, Jason S. Meyer, David M. Gamm, King-Wai Yau, M. Valeria Canto-Soler

School of Medicine

Research output: Contribution to journal › Article

Abstract

Many forms of blindness result from the dysfunction or loss of retinal photoreceptors. Induced pluripotent stem cells (iPSCs) hold great potential for the modelling of these diseases or as potential therapeutic agents. However, to fulfill this promise, a remaining challenge is to induce human iPSC to recreate in vitro key structural and functional features of the native retina, in particular the presence of photoreceptors with outer-segment discs and light sensitivity. Here we report that hiPSC can, in a highly autonomous manner, recapitulate spatiotemporally each of the main steps of retinal development observed in vivo and form three-dimensional retinal cups that contain all major retinal cell types arranged in their proper layers. Moreover, the photoreceptors in our hiPSC-derived retinal tissue achieve advanced maturation, showing the beginning of outer-segment disc formation and photosensitivity. This success brings us one step closer to the anticipated use of hiPSC for disease modelling and open possibilities for future therapies.

Original language	English (US)
Article number	4047
Journal	Nature Communications
Volume	5
DOIs	https://doi.org/10.1038/ncomms5047
Publication status	Published - Jun 10 2014

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ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Chemistry(all)
Physics and Astronomy(all)

Cite this

Zhong, X., Gutierrez, C., Xue, T., Hampton, C., Vergara, M. N., Cao, L. H., ... Canto-Soler, M. V. (2014). Generation of three-dimensional retinal tissue with functional photoreceptors from human iPSCs. Nature Communications, 5, [4047]. https://doi.org/10.1038/ncomms5047

Generation of three-dimensional retinal tissue with functional photoreceptors from human iPSCs. / Zhong, Xiufeng; Gutierrez, Christian; Xue, Tian; Hampton, Christopher; Vergara, M. Natalia; Cao, Li Hui; Peters, Ann; Park, Tea Soon; Zambidis, Elias; Meyer, Jason S.; Gamm, David M.; Yau, King-Wai; Canto-Soler, M. Valeria.

In: Nature Communications, Vol. 5, 4047, 10.06.2014.

Research output: Contribution to journal › Article

Zhong, Xiufeng ; Gutierrez, Christian ; Xue, Tian ; Hampton, Christopher ; Vergara, M. Natalia ; Cao, Li Hui ; Peters, Ann ; Park, Tea Soon ; Zambidis, Elias ; Meyer, Jason S. ; Gamm, David M. ; Yau, King-Wai ; Canto-Soler, M. Valeria. / Generation of three-dimensional retinal tissue with functional photoreceptors from human iPSCs. In: Nature Communications. 2014 ; Vol. 5.

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Access to Document

10.1038/ncomms5047