Generation of NY-ESO-1-specific CD4+ and CD8+ T cells by a single peptide with dual MHC class I and class II specificities: A new strategy for vaccine design

Gang Zeng, Yong Li, Mona El-Gamil, John Sidney, Alexandro Sette, Rong Fu Wang, Steven A. Rosenberg, Paul F. Robbins

Research output: Contribution to journalArticlepeer-review

Abstract

The existence of overlapping CD8+ and CD4+ T-cell epitopes within certain tumor antigens provides an opportunity to test the hypothesis that relatively short peptides could be used to generate both CD8+ and CD4+ T cells against tumor. In this report, T-cell responses to a fragment of the tumor antigen NY-ESO-1 that contained an HLA-DP4-restricted helper T cell epitope as well as an HLA-A2-restricted cytotoxic T cell epitope were analyzed. One peptide, ESO:157-170 (SLLMWITQCFLPVF) was recognized by both NY-ESO-1-reactive CD8+ and CD4+ T-cell clones. Both CD4+ and CD8+ T cells were efficiently generated from the peripheral blood of multiple melanoma patients after in vitro stimulations using ESO:157-170. Dual-specific peptides containing both cytotoxic T-cell and helper T-cell epitopes may represent an attractive strategy of vaccine design aimed at generating tumor-reactive CD4+ and CD8+ T cells.

Original languageEnglish (US)
Pages (from-to)3630-3635
Number of pages6
JournalCancer Research
Volume62
Issue number13
StatePublished - Jul 1 2002

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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