Generation of leukotrienes by purified human lung mast cells

Donald Macglashan, R. P. Schleimer, S. P. Peters, E. S. Schulman, G. K. Adams, H. H. Newball, L. M. Lichtenstein

Research output: Contribution to journalArticle

Abstract

Although mediator release from mast cells and basophils plays a central role in the pathogenesis of human allergic disease, biochemical studies have been restricted to rat peritoneal mast cells and basophilic leukemia cells because they could be easily purified. The authors have used two new techniques of cell separation to purify human lung mast cells to 98% homogeneity. Lung cell suspensions were obtained by dispersion of chopped lung tissue with proteolytic enzymes. Mast cells were then purified from the suspensions by countercurrent centrifugal elutriation and affinity chromatography. The purified mast cells released both histamine and slow-reacting substance of anaphylaxis (SRS-A) (leukotriene C and D) during stimulation with goat anti-human IgE antibody. Moreoever, these preparations were able to generate significant quantities of SRS-A (32 ± 7 x 10-17 LTD mole-equivalents/mast cell) at all stages of purification, indicating that a secondary cell is not necessary for the antigen-induced release of SRS.

Original languageEnglish (US)
Pages (from-to)747-751
Number of pages5
JournalJournal of Clinical Investigation
Volume70
Issue number4
StatePublished - 1982

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Leukotrienes
Mast Cells
Lung
SRS-A
Suspensions
Mast-Cell Leukemia
Leukotriene D4
Leukotriene C4
Basophils
Cell Separation
Affinity Chromatography
Goats
Histamine
Peptide Hydrolases
Antigens

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Macglashan, D., Schleimer, R. P., Peters, S. P., Schulman, E. S., Adams, G. K., Newball, H. H., & Lichtenstein, L. M. (1982). Generation of leukotrienes by purified human lung mast cells. Journal of Clinical Investigation, 70(4), 747-751.

Generation of leukotrienes by purified human lung mast cells. / Macglashan, Donald; Schleimer, R. P.; Peters, S. P.; Schulman, E. S.; Adams, G. K.; Newball, H. H.; Lichtenstein, L. M.

In: Journal of Clinical Investigation, Vol. 70, No. 4, 1982, p. 747-751.

Research output: Contribution to journalArticle

Macglashan, D, Schleimer, RP, Peters, SP, Schulman, ES, Adams, GK, Newball, HH & Lichtenstein, LM 1982, 'Generation of leukotrienes by purified human lung mast cells', Journal of Clinical Investigation, vol. 70, no. 4, pp. 747-751.
Macglashan D, Schleimer RP, Peters SP, Schulman ES, Adams GK, Newball HH et al. Generation of leukotrienes by purified human lung mast cells. Journal of Clinical Investigation. 1982;70(4):747-751.
Macglashan, Donald ; Schleimer, R. P. ; Peters, S. P. ; Schulman, E. S. ; Adams, G. K. ; Newball, H. H. ; Lichtenstein, L. M. / Generation of leukotrienes by purified human lung mast cells. In: Journal of Clinical Investigation. 1982 ; Vol. 70, No. 4. pp. 747-751.
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