Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.

Tomoyo Sawada; Kynon J.M. Benjamin; Anna C. Brandtjen; Ethan Tietze; Samuel J. Allen; Apuã C.M. Paquola; Joel E. Kleinman; Thomas M. Hyde; Jennifer A. Erwin

doi:10.1016/j.scr.2020.101806

Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.

Tomoyo Sawada, Kynon J.M. Benjamin, Anna C. Brandtjen, Ethan Tietze, Samuel J. Allen, Apuã C.M. Paquola, Joel E. Kleinman, Thomas M. Hyde, Jennifer A. Erwin

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using episomal vectors carrying OCT3/4, SOX2, KLF4, L-Myc, LIN28 and shRNA-p53. All iPS cell lines showed the same genotype with parental postmortem brain tissues, expressed pluripotency markers, and exhibited the differentiation potency into three embryonic germ layers.

Original language	English (US)
Article number	101806
Journal	Stem Cell Research
Volume	46
DOIs	https://doi.org/10.1016/j.scr.2020.101806
State	Published - Jul 2020

ASJC Scopus subject areas

Cell Biology
Developmental Biology

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10.1016/j.scr.2020.101806

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Sawada, T., Benjamin, K. J. M., Brandtjen, A. C., Tietze, E., Allen, S. J., Paquola, A. C. M., Kleinman, J. E., Hyde, T. M., & Erwin, J. A. (2020). Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium. Stem Cell Research, 46, Article 101806. https://doi.org/10.1016/j.scr.2020.101806

Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium. / Sawada, Tomoyo; Benjamin, Kynon J.M.; Brandtjen, Anna C. et al.
In: Stem Cell Research, Vol. 46, 101806, 07.2020.

Research output: Contribution to journal › Article › peer-review

Sawada, T, Benjamin, KJM, Brandtjen, AC, Tietze, E, Allen, SJ, Paquola, ACM, Kleinman, JE, Hyde, TM & Erwin, JA 2020, 'Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.', Stem Cell Research, vol. 46, 101806. https://doi.org/10.1016/j.scr.2020.101806

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title = "Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.",

abstract = "In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using episomal vectors carrying OCT3/4, SOX2, KLF4, L-Myc, LIN28 and shRNA-p53. All iPS cell lines showed the same genotype with parental postmortem brain tissues, expressed pluripotency markers, and exhibited the differentiation potency into three embryonic germ layers.",

author = "Tomoyo Sawada and Benjamin, {Kynon J.M.} and Brandtjen, {Anna C.} and Ethan Tietze and Allen, {Samuel J.} and Paquola, {Apu{\~a} C.M.} and Kleinman, {Joel E.} and Hyde, {Thomas M.} and Erwin, {Jennifer A.}",

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year = "2020",

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doi = "10.1016/j.scr.2020.101806",

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AU - Brandtjen, Anna C.

AU - Tietze, Ethan

AU - Allen, Samuel J.

AU - Paquola, Apuã C.M.

AU - Kleinman, Joel E.

AU - Hyde, Thomas M.

AU - Erwin, Jennifer A.

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AB - In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using episomal vectors carrying OCT3/4, SOX2, KLF4, L-Myc, LIN28 and shRNA-p53. All iPS cell lines showed the same genotype with parental postmortem brain tissues, expressed pluripotency markers, and exhibited the differentiation potency into three embryonic germ layers.

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Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.

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