TY - JOUR
T1 - Generation of a variant of human interleukin-4 by alternative splicing
AU - Alms, William J.
AU - Atamas, Sergei P.
AU - Yurovsky, Vladimir V.
AU - White, Barbara
PY - 1996/3
Y1 - 1996/3
N2 - A second species of interleukin-4 (IL-4) mRNA was identified using both a reverse transcription polymerase chain reaction and an RNase protection assay. This novel IL-4 mRNA was 48 base pairs smaller than IL-4 mRNA, which is the size of IL-4 exon 2. Sequence data of cloned cDNA demonstrated that this variant contained IL-4 exons 1, 3 and 4, with exon 1 spliced directly to exon 3 in an open reading frame. The entire protein encoding region of this variant, named IL-4δ2, was identical to IL-4 except for the omission of exon 2. IL-4δ2 mRNA was detected in all human peripheral blood mononuclear cells tested and in purified CD3+ T cells. Amounts of both IL-4 and IL-4δ2 mRNAs increased upon T cell activation, although IL-4 mRNA increased to a greater extent than IL-4δ2 mRNA did. Human IL-3, IL-5, IL-13, and granulocyte macrophage-colony stimulating factor did not use alternative splicing to delete exon 2. We speculate that IL-4δ2 may regulate IL-4 function.
AB - A second species of interleukin-4 (IL-4) mRNA was identified using both a reverse transcription polymerase chain reaction and an RNase protection assay. This novel IL-4 mRNA was 48 base pairs smaller than IL-4 mRNA, which is the size of IL-4 exon 2. Sequence data of cloned cDNA demonstrated that this variant contained IL-4 exons 1, 3 and 4, with exon 1 spliced directly to exon 3 in an open reading frame. The entire protein encoding region of this variant, named IL-4δ2, was identical to IL-4 except for the omission of exon 2. IL-4δ2 mRNA was detected in all human peripheral blood mononuclear cells tested and in purified CD3+ T cells. Amounts of both IL-4 and IL-4δ2 mRNAs increased upon T cell activation, although IL-4 mRNA increased to a greater extent than IL-4δ2 mRNA did. Human IL-3, IL-5, IL-13, and granulocyte macrophage-colony stimulating factor did not use alternative splicing to delete exon 2. We speculate that IL-4δ2 may regulate IL-4 function.
KW - alternative spicing
KW - Interleukin-4
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UR - http://www.scopus.com/inward/citedby.url?scp=0029994459&partnerID=8YFLogxK
U2 - 10.1016/0161-5890(95)00154-9
DO - 10.1016/0161-5890(95)00154-9
M3 - Article
C2 - 8676887
AN - SCOPUS:0029994459
SN - 0161-5890
VL - 33
SP - 361
EP - 370
JO - Molecular Immunology
JF - Molecular Immunology
IS - 4-5
ER -