@inproceedings{98337b265d8d4f1c82b51ddf9ccda427,
title = "Generation of a conditional analogsensitive kinase in human cells using CRISPR/Cas9-mediated genome engineering",
abstract = "The ability to rapidly and specifically modify the genome of mammalian cells has been a long-term goal of biomedical researchers. Recently, the clustered, regularly interspaced, short palindromic repeats (CRISPR)/Cas9 system from bacteria has been exploited for genome engineering in human cells. The CRISPR system directs the RNA-guided Cas9 nuclease to a specific genomic locus to induce a DNA double-strand break that may be subsequently repaired by homology-directed repair using an exogenous DNA repair template. Here we describe a protocol using CRISPR/Cas9 to achieve bi-allelic insertion of a point mutation in human cells. Using this method, homozygous clonal cell lines can be constructed in 5e6 weeks. This method can also be adapted to insert larger DNA elements, such as fluorescent proteins and degrons, at defined genomic locations. CRISPR/Cas9 genome engineering offers exciting applications in both basic science and translational research.",
author = "Moyer, {Tyler C.} and Holland, {Andrew J.}",
note = "Publisher Copyright: {\textcopyright} 2015 Elsevier Inc.",
year = "2015",
doi = "10.1016/bs.mcb.2015.03.017",
language = "English (US)",
isbn = "9780128024492",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "19--36",
editor = "Karen Oegema and Renata Basto",
booktitle = "Methods in Cell Biology",
}