Generation and molecular cytogenetic characterization of a radiation-reduction hybrid panel for human chromosome 6

G. J. Pappas, E. Thompson, A. Burgess, A. Greenwood, J. M. Trent

Research output: Contribution to journalArticlepeer-review

Abstract

A neoR marked chromosome-6 containing hybrid (D113 JA) was used to generate a panel of 15 radiation-reduced hybrid cell lines. The panel was constructed by irradiating microcells isolated from D113JA at 800 or 8000 rads, providing different levels of chromosome 6 retention. These hybrids have been systematically analyzed using interspersed repetitive elements, previously assigned markers for chromosome 6, and fluorescent in situ hybridization (FISH). As expected, G418 selection has favored the retention of fragments near the insertion site of the neoR gene (6q16). The panel as constituted provides an important resource for regional assignment of molecular markers, especially to regions on 6q.

Original languageEnglish (US)
Pages (from-to)201-206
Number of pages6
JournalCytogenetic and Genome Research
Volume69
Issue number3-4
DOIs
StatePublished - 1995

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Fingerprint Dive into the research topics of 'Generation and molecular cytogenetic characterization of a radiation-reduction hybrid panel for human chromosome 6'. Together they form a unique fingerprint.

Cite this