Abstract
Human embryonic stem (ES) cells are capable of unlimited proliferation and maintenance of pluripotency in vitro; these properties may lead to potential applications in regenerative medicine. However, immune rejection hampers the allogenic application of human ES cells. Over-expression of several specific transcription factors has been used to reprogram human adult cells into induced pluripotent stem (iPS) cells, which are similar to hESCs in many aspects. The iPS technique makes it possible to produce patient-specific pluripotent stem cells for transplantation therapy without immune rejection. However, some challenges remain, including viral vector integration into the genome, the existence of exogenous oncogenic factors, and low induction efficiency. Here, we review recent advances in human iPS methodology, as well as remaining challenges and its potential applications.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 9-13 |
| Number of pages | 5 |
| Journal | Chinese Science Bulletin |
| Volume | 54 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2009 |
Fingerprint
Keywords
- Differentiation
- ES cell
- iPS
- Regenerative medicine
- Reprogramming
- Transplantation
ASJC Scopus subject areas
- General
Cite this
Generation and application of human iPS cells. / Cui, Chun; Rao, LingJun; Cheng, Linzhao; Xiao, Lei.
In: Chinese Science Bulletin, Vol. 54, No. 1, 01.2009, p. 9-13.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Generation and application of human iPS cells
AU - Cui, Chun
AU - Rao, LingJun
AU - Cheng, Linzhao
AU - Xiao, Lei
PY - 2009/1
Y1 - 2009/1
N2 - Human embryonic stem (ES) cells are capable of unlimited proliferation and maintenance of pluripotency in vitro; these properties may lead to potential applications in regenerative medicine. However, immune rejection hampers the allogenic application of human ES cells. Over-expression of several specific transcription factors has been used to reprogram human adult cells into induced pluripotent stem (iPS) cells, which are similar to hESCs in many aspects. The iPS technique makes it possible to produce patient-specific pluripotent stem cells for transplantation therapy without immune rejection. However, some challenges remain, including viral vector integration into the genome, the existence of exogenous oncogenic factors, and low induction efficiency. Here, we review recent advances in human iPS methodology, as well as remaining challenges and its potential applications.
AB - Human embryonic stem (ES) cells are capable of unlimited proliferation and maintenance of pluripotency in vitro; these properties may lead to potential applications in regenerative medicine. However, immune rejection hampers the allogenic application of human ES cells. Over-expression of several specific transcription factors has been used to reprogram human adult cells into induced pluripotent stem (iPS) cells, which are similar to hESCs in many aspects. The iPS technique makes it possible to produce patient-specific pluripotent stem cells for transplantation therapy without immune rejection. However, some challenges remain, including viral vector integration into the genome, the existence of exogenous oncogenic factors, and low induction efficiency. Here, we review recent advances in human iPS methodology, as well as remaining challenges and its potential applications.
KW - Differentiation
KW - ES cell
KW - iPS
KW - Regenerative medicine
KW - Reprogramming
KW - Transplantation
UR - http://www.scopus.com/inward/record.url?scp=58149462270&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149462270&partnerID=8YFLogxK
U2 - 10.1007/s11434-008-0579-8
DO - 10.1007/s11434-008-0579-8
M3 - Article
AN - SCOPUS:58149462270
VL - 54
SP - 9
EP - 13
JO - Science Bulletin
JF - Science Bulletin
SN - 2095-9273
IS - 1
ER -