Generation and application of human iPS cells

Chun Cui, LingJun Rao, Linzhao Cheng, Lei Xiao

Research output: Contribution to journalArticle

Abstract

Human embryonic stem (ES) cells are capable of unlimited proliferation and maintenance of pluripotency in vitro; these properties may lead to potential applications in regenerative medicine. However, immune rejection hampers the allogenic application of human ES cells. Over-expression of several specific transcription factors has been used to reprogram human adult cells into induced pluripotent stem (iPS) cells, which are similar to hESCs in many aspects. The iPS technique makes it possible to produce patient-specific pluripotent stem cells for transplantation therapy without immune rejection. However, some challenges remain, including viral vector integration into the genome, the existence of exogenous oncogenic factors, and low induction efficiency. Here, we review recent advances in human iPS methodology, as well as remaining challenges and its potential applications.

Original languageEnglish (US)
Pages (from-to)9-13
Number of pages5
JournalChinese Science Bulletin
Volume54
Issue number1
DOIs
StatePublished - Jan 2009

Fingerprint

Induced Pluripotent Stem Cells
Virus Integration
Pluripotent Stem Cells
Regenerative Medicine
Stem Cell Transplantation
Cell- and Tissue-Based Therapy
Transcription Factors
Maintenance
Genome
Human Embryonic Stem Cells

Keywords

  • Differentiation
  • ES cell
  • iPS
  • Regenerative medicine
  • Reprogramming
  • Transplantation

ASJC Scopus subject areas

  • General

Cite this

Generation and application of human iPS cells. / Cui, Chun; Rao, LingJun; Cheng, Linzhao; Xiao, Lei.

In: Chinese Science Bulletin, Vol. 54, No. 1, 01.2009, p. 9-13.

Research output: Contribution to journalArticle

Cui, Chun ; Rao, LingJun ; Cheng, Linzhao ; Xiao, Lei. / Generation and application of human iPS cells. In: Chinese Science Bulletin. 2009 ; Vol. 54, No. 1. pp. 9-13.
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