Generating renal cancer-reactive T cells using dendritic cells (DCs) to present autologous tumor

Qiong J. Wang, Ken Ichi Hanada, Donna Perry-Lalley, Maria P. Bettinotti, Tatiana Karpova, Hung T. Khong, James C. Yang

Research output: Contribution to journalArticlepeer-review

Abstract

Dendritic cells (DCs) have been used as professional antigen-presenting cells in vitro to prime T-cell responses. In this study, we generated both CD8+ and CD4+ renal cell carcinoma (RCC)-reactive T cells using a completely autologous system of DCs presenting engulfed whole-tumor cells. We compared DCs presenting RCC tumor cells in different preparations and found ultraviolet-irradiated apoptotic tumor cells to be more immunogenic than necrotic tumor cells or live untreated tumor cells in generating tumor-reactive T cells. In analyzing the T cells generated in this fashion, a CD8+ RCC-reactive T-cell clone generated from a patient recognized an epitope derived from fibroblast growth factor 5 in the context of human leukocyte antigen (HLA) B44*02. CD4+ T cells generated from another patient recognized multiple allogeneic RCC lines expressing HLA-DRβ1*04, indicating a common shared tumor antigen presented by HLA-DRβ1*04. The technique of using DCs to present whole-tumor cells can consistently generate both CD4+ and CD8+ RCC-reactive T cells for use in both antigen identification and therapeutic protocols.

Original languageEnglish (US)
Pages (from-to)551-559
Number of pages9
JournalJournal of Immunotherapy
Volume28
Issue number6
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

Keywords

  • Apoptosis
  • Dendritic cells
  • Necrosis
  • Renal cell carcinoma
  • T cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology
  • Cancer Research

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