Generating mammalian stable cell lines by electroporation

Patti A. Longo, Jennifer M. Kavran, Min Sung Kim, Daniel J. Leahy

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Expression of functional, recombinant mammalian proteins often requires expression in mammalian cells (see Single Cell Cloning of a Stable Mammalian Cell Line). If the expressed protein needs to be made frequently, it can be best to generate a stable cell line instead of performing repeated transient transfections into mammalian cells. Here, we describe a method to generate stable cell lines via electroporation followed by selection steps. This protocol will be limited to the CHO dhfr- Urlaub et al. (1983) and LEC1 cell lines, which in our experience perform the best with this method.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationDNa
PublisherAcademic Press Inc.
Pages209-226
Number of pages18
ISBN (Print)9780124186873
DOIs
StatePublished - 2013

Publication series

NameMethods in Enzymology
Volume529
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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