TY - JOUR
T1 - Generating chimeric spinal cord
T2 - a novel model for transplantable oligodendrocyte progenitors derived from embryonic stem cells.
AU - Vadivelu, Sudhakar
AU - Becker, Daniel
AU - McDonald, John W.
PY - 2005
Y1 - 2005
N2 - OBJECT: To identify and evaluate stem cell-derived oligodendrocytes obtained for cell transplantation therapies, the authors developed a novel model to examine single, adult oligodendrocytes in situ. METHODS: Green fluorescent protein-expressing, mouse embryonic stem cells (ESCs) were neural induced and additionally staged in an oligosphere preparatory step for high-yield derivation of oligodendrocyte progenitors. These transplantable, induced progenitors were injected into postnatal Day 2 rat pups, in which spinal cord sections were then examined at 3 and 9 weeks posttransplantation. CONCLUSIONS: Transplanted oligosphere ESCs survived and integrated anatomically into postnatal and adult white matter, generating targeted regions of chimeric spinal cord. A simple model for identifying adult oligodendrocytes in situ is presented, which is suitable for use in further studies examining functional myelination and derivation of oligodendrocytes from genetically engineered ESC lines, including human ESCs. Results from the model presented here demonstrate a unique method for examining transplantable oligodendrocyte progenitors derived from ESCs for repair of white matter disease.
AB - OBJECT: To identify and evaluate stem cell-derived oligodendrocytes obtained for cell transplantation therapies, the authors developed a novel model to examine single, adult oligodendrocytes in situ. METHODS: Green fluorescent protein-expressing, mouse embryonic stem cells (ESCs) were neural induced and additionally staged in an oligosphere preparatory step for high-yield derivation of oligodendrocyte progenitors. These transplantable, induced progenitors were injected into postnatal Day 2 rat pups, in which spinal cord sections were then examined at 3 and 9 weeks posttransplantation. CONCLUSIONS: Transplanted oligosphere ESCs survived and integrated anatomically into postnatal and adult white matter, generating targeted regions of chimeric spinal cord. A simple model for identifying adult oligodendrocytes in situ is presented, which is suitable for use in further studies examining functional myelination and derivation of oligodendrocytes from genetically engineered ESC lines, including human ESCs. Results from the model presented here demonstrate a unique method for examining transplantable oligodendrocyte progenitors derived from ESCs for repair of white matter disease.
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U2 - 10.3171/foc.2005.19.3.4
DO - 10.3171/foc.2005.19.3.4
M3 - Article
C2 - 16190602
AN - SCOPUS:33646105654
SN - 1092-0684
VL - 19
SP - E3
JO - Neurosurgical focus
JF - Neurosurgical focus
IS - 3
ER -